Endoglycosidase H Enzyme
Endoglycosidase H (Endo-b-N-acetylglucosaminidase H)
Purity
Molecular Biology GradeEndoglycosidase H was purified from the culture fluid of Streptococcus griseus using DEAE-Sephadex and Sephadex G-75.
Synonyms
Endoglycosidase H; Endoglycosidase H (Endo-b-N-acetylglucosaminidase H); Endoglycosidase H enzyme
Host
Streptococcus griseus
Purity/Purification
Molecular Biology Grade
Endoglycosidase H was purified from the culture fluid of Streptococcus griseus using DEAE-Sephadex and Sephadex G-75.
Endoglycosidase H was purified from the culture fluid of Streptococcus griseus using DEAE-Sephadex and Sephadex G-75.
Form/Format
Supplied as a lyophilized powder from 10mM Tris-HCl, pH7.2 Reconstitute with 0.1ml sterile, distilled, deionized water.
Appearance: Lyophilized powder
Appearance: Lyophilized powder
Activity
0.1units
Protein: 6ug/vial
Specific Activity:
~30 units/mg protein
Protein: 6ug/vial
Specific Activity:
~30 units/mg protein
Contaminants
No detectable amount of various exoglycosidases or proteases
Quality Control
Endoglycosidase H (0.01 unit) was incubated with appropriate p-nitrophenylglycosides (0.1mg) in 0.2ml of citrate-phosphate buffer (pH 5.0) at 37 degree C for 12 hours. No activites of a- and b-galactosidase or a-L-fucosidase were observed. Absence of contaminating a-mannosidase was also confirmed by using oligosaccharides from ovalbumin and yeast mannan.
Endoglycosidase H (0.01 unit) was incubated with 10mg casein in 1ml of 0.1M Tris-CHl buffer at 37 degree C for 20 hours. 1ml of 10% TCA was added. Release of acid soluble material was below the limit of detection (OD275 less than 0.05).
To inhibit potential protease activity during extended Endo-H digestions, 1mM PMSF and/or 10mM pepstatin A may be added to incubations. PMSF is stable as a 2mM stock solution in absolute methanol.
Endoglycosidase H (0.01 unit) was incubated with 10mg casein in 1ml of 0.1M Tris-CHl buffer at 37 degree C for 20 hours. 1ml of 10% TCA was added. Release of acid soluble material was below the limit of detection (OD275 less than 0.05).
To inhibit potential protease activity during extended Endo-H digestions, 1mM PMSF and/or 10mM pepstatin A may be added to incubations. PMSF is stable as a 2mM stock solution in absolute methanol.
Unit Definition
One unit is that amount of enzyme required to hydrolyze 1mmole of (Man)5-(GlcNAc)2Asn-[14C]-acetyl, at pH 5.0 and 37 degree C per minute at the saturated substrate concentration. Muramatsu's method is employed).
Preparation and Storage
-20 degree C
Stability: Stable at pH 5.0-9.0 at 37 degree C for 20 hours. Stable for 2 years at -20 degree C. Aliquots of dissolved enzymes may be stored at -20 degree C. Repeated freezing and thawing may decrease the enzyme acitivity.
Stability: Stable at pH 5.0-9.0 at 37 degree C for 20 hours. Stable for 2 years at -20 degree C. Aliquots of dissolved enzymes may be stored at -20 degree C. Repeated freezing and thawing may decrease the enzyme acitivity.
Related Product Information for Endoglycosidase H enzyme
Suitable for studies of glycopeptide and glycoprotein with "High Mannose" sugar chains.
Product Categories/Family for Endoglycosidase H enzyme
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Endo-beta-N-acetylglucosaminidase H
Uniprot Description
Cleaves asparagine-linked oligomannose and hybrid, but not complex, oligosaccharides from glycoproteins.
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Product Notes
The Endoglycosidase H (Catalog #AAA653913) is an Enzyme produced from Streptococcus griseus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Endoglycosidase H, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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