STK3 recombinant protein

STK3, Active

Cat. Num. AAA517581

• Gene Names: STK3; KRS1; MST2
• Applications: Western Blot
• Synonyms: STK3; Active; KRS1; MST2; FLJ90748; STK3 recombinant protein

• Host: Sf9 insect cells
• Form/Format: Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

• Applicable Applications for STK3 recombinant protein: Kinase Assay, Western Blot (WB)
• Tag: GST
• Species: Human
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Activity

(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)

SDS-Page

(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

Related Product Information for STK3 recombinant protein

Recombinant full-length human STK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is BC010640.
STK3, also known as MST2, encodes a protein of 491-amino acid which contains an N-terminal catalytic domain characteristic of STKs (1). STK3 and STK4 share 94% amino acid identity in the catalytic domain and 78% identity overall. RAF1 has been shown to counteract apoptosis by suppressing the activation of mammalian sterile 20-like kinase (MST2). STK3 is a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (2). STK3 activation presumably allows cells to resist unfavorable environmental conditions.

Product Categories/Family for STK3 recombinant protein

Protein Kinases; AKT/PKB Pathway; Apoptosis/Autophagy; Cancer; Cardiovascular Disease; Inflammation; JNK/SAPK Pathway; Neurobiology; Ser/Thr Kinases

References

1. Creasy, C L. et al: Cloning and characterization of a member of the MST subfamily of Ste20-like kinases. Gene 167: 303-306, 1995.
2. Lee, K K. et al: MST, a physiological caspase substrate, higly sensitizes apoptosis both upstream and downstream of caspase activation. J. Biol. Chem. 276: 19276-19285, 2001.

NCBI and Uniprot Product Information

• NCBI GI #: 34189966
• NCBI GeneID: 6788
• Molecular Weight: 59,462 Da
• NCBI Official Full Name: Homo sapiens serine/threonine kinase 3 (STE20 homolog, yeast), mRNA
• NCBI Official Synonym Full Names: serine/threonine kinase 3
• NCBI Official Symbol: STK3
• NCBI Official Synonym Symbols: KRS1; MST2
• NCBI Protein Information: serine/threonine-protein kinase 3
• Protein Family: Serine/threonine-protein kinase

NCBI Description

This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]