SOK1 recombinant protein

SOK1, Active

Cat. Num. AAA515689

• Gene Names: STK25; SOK1; YSK1
• Applications: Western Blot
• Synonyms: SOK1; Active; SOK1 recombinant protein

• Host: Sf9 insect cells using baculovirus
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Positions: Full Length
• Sequence Length: 2527

• Applicable Applications for SOK1 recombinant protein: Kinase Assay, Western Blot (WB)
• Species: Human
• Tag: GST tag
• Preparation and Storage: Store product at -70 degree C.; For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.; For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Testing Data

Testing Data #2

Related Product Information for SOK1 recombinant protein

Description: Full-length recombinant human SOK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: SOK1 or oxidant stress-response kinase-1 is a serine/threonine kinase that is activated by autophosphorylation and by reactive oxygen intermediates, but is not activated by growth factors, alkylating agents, cytokines, or environmental stresses (1). SOK1 is expressed ubiquitously with highest expression in brain and testis. SOK1 regulates cell death after chemical anoxia, as its down-regulation by RNA interference enhances cell survival. SOK1 entry into the nucleus is important for the cell death response since SOK1 mutants unable to enter the nucleus do not induce cell death (2).

Product Categories/Family for SOK1 recombinant protein

Enzymes; Active Kinases

References

1. Pombo, C M. et al: Activation of a human Ste20-like kinase by oxidant stress defines a novel stress response pathway. EMBO J. 15: 4537-4546, 1996. 2. Nogueira, E. et al: SOK1 translocates from the Golgi to the nucleus upon chemical anoxia and induces apoptotic cell death. J Biol Chem. 2008 Jun 6; 283 (23):16248-58.

NCBI and Uniprot Product Information

• NCBI GI #: 433282979
• NCBI GeneID: 10494
• NCBI Accession #: NM_006374.4
• NCBI GenBank Nucleotide #: NM_006374
• UniProt Accession #: O00506; Q15522; Q5BJF1; A8K6Z3; A8K7D2; B7Z9K1
• Molecular Weight: ~76 kDa
• NCBI Official Full Name: Homo sapiens serine/threonine kinase 25 (STK25), transcript variant 4, mRNA
• NCBI Official Synonym Full Names: serine/threonine kinase 25
• NCBI Official Symbol: STK25
• NCBI Official Synonym Symbols: SOK1; YSK1
• NCBI Protein Information: serine/threonine-protein kinase 25
• UniProt Protein Name: Serine/threonine-protein kinase 25
• Protein Family: Protein
• UniProt Gene Name: STK25
• UniProt Synonym Gene Names: SOK1; YSK1; SOK-1; Ste20/oxidant stress response kinase 1

NCBI Description

This gene encodes a member of the germinal centre kinase III (GCK III) subfamily of the sterile 20 superfamily of kinases. The encoded enzyme plays a role in serine-threonine liver kinase B1 (LKB1) signaling pathway to regulate neuronal polarization and morphology of the Golgi apparatus. The protein is translocated from the Golgi apparatus to the nucleus in response to chemical anoxia and plays a role in regulation of cell death. A pseudogene associated with this gene is located on chromosome 18. Multiple alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

YSK1: Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; YSK subfamily

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoplasm; Golgi apparatus

Molecular Function: MAP kinase kinase kinase kinase activity; protein binding; protein homodimerization activity; protein kinase activity

Biological Process: establishment of Golgi localization; Golgi localization; neurite morphogenesis; positive regulation of stress-activated MAPK cascade; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of apoptosis; regulation of mitotic cell cycle; response to hydrogen peroxide; response to oxidative stress; signal transduction