Human Stress Induced Phosphoprotein 1 ELISA Kit | STIP1 elisa kit

Human Stress Induced Phosphoprotein 1 ELISA Kit

Cat. Num. AAA95979

• Gene Names: STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
• Reactivity: Human
• Synonyms: Stress Induced Phosphoprotein 1; Human Stress Induced Phosphoprotein 1 ELISA Kit; STIP1 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human STIP1 and analogues was observed.
• Sequence Length: 543

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 3.12ng/ml-100ng/ml.
• Sensitivity: 1.0ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated STIP1 concentrations in Human serum, plasma and other body fluids. Using Purified Human STIP1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add STIP1 and STIP1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of STIP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for STIP1 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated STIP1 concentrations in Human serum, plasma and other body fluids. Using Purified Human STIP1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add STIP1 and STIP1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of STIP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5803181
• NCBI GeneID: 10963
• NCBI Accession #: NP_006810.1
• NCBI GenBank Nucleotide #: NM_006819.2
• UniProt Accession #: P31948; Q3ZCU9; Q5TZU9; B4DM70; F5H0T1; G3XAD8
• Molecular Weight: 62,639 Da
• NCBI Official Full Name: stress-induced-phosphoprotein 1 isoform b
• NCBI Official Synonym Full Names: stress-induced phosphoprotein 1
• NCBI Official Symbol: STIP1
• NCBI Official Synonym Symbols: HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
• NCBI Protein Information: stress-induced-phosphoprotein 1; NY-REN-11 antigen; Hsp70/Hsp90-organizing protein; hsc70/Hsp90-organizing protein; renal carcinoma antigen NY-REN-11; transformation-sensitive protein IEF SSP 3521; epididymis secretory sperm binding protein Li 94n
• UniProt Protein Name: Stress-induced-phosphoprotein 1
• UniProt Gene Name: STIP1
• UniProt Synonym Gene Names: STI1; Hop
• UniProt Entry Name: STIP1_HUMAN

NCBI Description

STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM, Jul 2009]

Uniprot Description

STI1: a protein that is apparently involved in the response to stress. Specifically interacts with the C-terminal tails of Hsp70 and Hsp90 via TPR1 and TPR2A domains. Deletion of STI1 reduces the activity of the glucocorticoid receptor, a Hsp90 target protein. Phosphorylated in vitro by p90RSK and casein kinase II.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: Golgi apparatus; nucleus

Molecular Function: protein binding

Biological Process: response to stress