Rat Steroidogenic Acute Regulatory Protein ELISA Kit | STAR elisa kit

Rat Steroidogenic Acute Regulatory Protein ELISA Kit

Cat. Num. AAA6463

• Gene Names: STAR; STARD1
• Reactivity: Rat
• Synonyms: Steroidogenic Acute Regulatory Protein; Rat Steroidogenic Acute Regulatory Protein ELISA Kit; STAR elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Rat STAR and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 31.2mumol/L-1000mumol/L.
• Sensitivity: 5.0mumol/L.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated STAR concentrations in Rat serum, plasma and other body fluids. Using Purified Rat STAR antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add STAR and STAR antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of STAR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for STAR elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated STAR concentrations in Rat serum, plasma and other body fluids. Using Purified Rat STAR antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add STAR and STAR antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of STAR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 49456655
• NCBI GeneID: 6770
• UniProt Accession #: Q6IBK0; P49675
• Molecular Weight: 31,914 Da
• NCBI Official Full Name: STAR
• NCBI Official Synonym Full Names: steroidogenic acute regulatory protein
• NCBI Official Symbol: STAR
• NCBI Official Synonym Symbols: STARD1
• NCBI Protein Information: steroidogenic acute regulatory protein, mitochondrial; cholesterol trafficker; START domain containing 1; steroid acute regulatory protein; START domain-containing protein 1; mitochondrial steroid acute regulatory protein; StAR-related lipid transfer (START) domain containing 1
• UniProt Protein Name: STAR protein
• Protein Family: Steroidogenic acute regulatory protein
• UniProt Gene Name: STAR
• UniProt Entry Name: Q6IBK0_HUMAN

NCBI Description

The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. [provided by RefSeq, Jul 2008]

Uniprot Description

STAR: Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone. May interact with TSPO. Expressed in gonads, adrenal cortex and kidney.

Protein type: Mitochondrial; Carrier; Lipid-binding

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: mitochondrial crista; neuron projection; cell soma; mitochondrial intermembrane space; cytosol

Molecular Function: cholesterol transporter activity; cholesterol binding

Biological Process: response to nicotine; steroid metabolic process; diterpenoid metabolic process; dibenzo-p-dioxin metabolic process; regulation of steroid biosynthetic process; estrogen biosynthetic process; biphenyl metabolic process; circadian sleep/wake cycle, REM sleep; negative regulation of neuron apoptosis; response to nutrient; response to corticosterone stimulus; response to drug; bile acid biosynthetic process; cholesterol metabolic process; positive regulation of neurogenesis; progesterone biosynthetic process; insecticide metabolic process; fractalkine metabolic process; male gonad development; phthalate metabolic process; intracellular cholesterol transport; response to herbicide; glucocorticoid metabolic process; response to ethanol; cellular response to insulin stimulus; response to hydrogen peroxide; response to estrogen stimulus; response to lead ion; C21-steroid hormone biosynthetic process; response to ionizing radiation; brain development; response to activity; steroid biosynthetic process; regulation of neuronal synaptic plasticity; phenol metabolic process

Disease: Lipoid Congenital Adrenal Hyperplasia