Protease, S. aureus Enzyme | MW1399 enzyme
Protease, S. aureus (Endoproteinase Glu C)
Purity
Non-SequencingChromatographically purified
Synonyms
Protease; S. aureus; S. aureus (Endoproteinase Glu C); MW1399 enzyme
Host
Staphylcoccus aureus strain V8
Purity/Purification
Non-Sequencing
Chromatographically purified
Chromatographically purified
Form/Format
Supplied as a lyophilized powder
Activity
500 units per mg dry weight
Extinction Coefficient
4.26
Quality Control
SDS-PAGE
Unit Definition
One Unit causes a change of 0.001at A280nm per minute at 37 degree C, pH 7.8 using casein as the substrate.
Preparation and Storage
Autolysis occurs at temperatures greater than 40 degree C. The enzyme is fully active in 2mg/ml SDS. Stable for 6 months at 4 degree C.
Related Product Information for MW1399 enzyme
Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976).
Product Categories/Family for MW1399 enzyme
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27kD
NCBI Official Full Name
protease
NCBI Official Symbol
MW1399
NCBI Protein Information
protease
UniProt Protein Name
Protease
UniProt Entry Name
Q8NWJ9_STAAW
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Product Notes
The MW1399 (Catalog #AAA653731) is an Enzyme produced from Staphylcoccus aureus strain V8 and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Protease, S. aureus, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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