Testing Data
(Human PBMC were stained with FoxP3/Transcription Factor staining kit (17-1014). Anti-CD4 PE and anti-CD25 APC positive cells were gated, further analyzed in anti-FoxP3 FITC using 2 ug antibodies. Green represents FITC conjugated Isotope control . Red represents anti-FoxP3 FITC.)
Foxp3/Transcription Factor Staining Kit | FOXP3 staining kit
Foxp3/Transcription Factor Staining Kit
Testing Data
(Human PBMC were stained with FoxP3/Transcription Factor staining kit (17-1014). Anti-CD4 PE and anti-CD25 APC positive cells were gated, further analyzed in anti-FoxP3 FITC using 2 ug antibodies. Green represents FITC conjugated Isotope control . Red represents anti-FoxP3 FITC.)
Testing Data
(Human PBMC were stained with FoxP3/Transcription Factor staining kit (17-1014). Anti-CD4 PE and anti-CD25 APC positive cells were gated, further analyzed in anti-FoxP3 FITC using 2 ug antibodies. Green represents FITC conjugated Isotope control . Red represents anti-FoxP3 FITC.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
FOXP3: Probable transcription factor. Plays a critical role in the control of immune response. Interacts with IKZF3. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: C2H2-type zinc finger protein; Cell cycle regulation; DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: Xp11.23
Cellular Component: cytoplasm; nucleus
Molecular Function: histone acetyltransferase binding; histone deacetylase binding; NFAT protein binding; protein binding; protein homodimerization activity; sequence-specific DNA binding; transcription factor activity
Biological Process: CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment; inhibition of CREB transcription factor; inhibition of NF-kappaB transcription factor; negative regulation of cell proliferation; negative regulation of cytokine biosynthetic process; negative regulation of cytokine secretion; negative regulation of histone deacetylation; negative regulation of immune response; negative regulation of interferon-gamma production; negative regulation of interleukin-10 production; negative regulation of interleukin-17 production; negative regulation of interleukin-2 biosynthetic process; negative regulation of interleukin-2 production; negative regulation of interleukin-4 production; negative regulation of T cell cytokine production; negative regulation of T cell proliferation; negative regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of T cell anergy; response to virus; T cell activation
Disease: Diabetes Mellitus, Insulin-dependent; Immunodysregulation, Polyendocrinopathy, And Enteropathy, X-linked
Research Articles on FOXP3
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Product Notes
The FOXP3 foxp3 (Catalog #AAA668928) is a Staining Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Foxp3/Transcription Factor can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Protocol: FoxP3 FITC staining with CD4 PE and CD25 APC antibodies. 1. Determine number of cells required for staining. Each sample contains 0.5 -1 x 10^6 cells in 50ul of media or staining buffer. The following controls are needed for the experiment. Unstained cells (no antibodies were added), cells with isotype control and cells with secondary antibody (if secondary antibody was used). 2. Centrifuge cells at 1000 RPM for 10 minutes and decant supernatant. 3. Resuspend cells with appropriate volume of staining buffer. 4. Aliquote 1x10^6 cells in 50 ul to the desired number of flow tubes. Dilute anti-CD4 PE, anti-CD25 APC cell surface antibodies in 50 ul of 1X staining buffer. Add diluted antibodies into 50 ul of cell suspension. Mix antibodies in cells suspension thoroughly. 5. Incubate in ice for 30 minutes in ice pritected from light. Wash cells in 2-3 ml of 1X staining buffer. Centrifuge 1000 RPM for 10 minutes. Decant supernatant carefully. 6. Add 1ml of freshly prepared 1X fixation/permeabilization buffer. Mix well. 7. Incubate in ice for 30 minutes protected from light. 8. Whsh cells in 3-4 ml of 1X permeabilization buffer. Centrifuge at 1000 RPM for 10 minutes. Decant supernatant carefully. 9. Add 2% mouse serum to the cells in 50 ul of 1X permeabilization buffer (for blocking non-specific binding). Incubate 10 minutes in ice. (Optional) 10. Add fluorochrome conjugated Anti-FoxP3 antibody or isotype control in 50 ul of 1X permeabilization buffer. (Without washing blocking step). 11. Incubate in ice for 30 minutes, protected from light. Wash cells 3-4 ml of 1X permeabilization buffer. Centrifuge 1000 RPM for 10 minutes. Decant supernatant carefully. 11. After decanting, add 300-400ul of staining buffer to each tube. If not analyzing same day, samples can be stored over night in dark at 4 degrees C. Samples can be analyzed in Flow Cytometer according to the manufacturer protocol. Researchers should empirically determine the suitability of the FOXP3 foxp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Foxp3/Transcription Factor, Staining Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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