SFRS2 Blocking Peptide | SRSF2 blocking peptide

SFRS2 Antibody (Center) Blocking Peptide

• Gene Names: SRSF2; SC35; PR264; SC-35; SFRS2; SFRS2A; SRp30b
• Synonyms: SFRS2; SFRS2 Antibody (Center) Blocking Peptide; Serine/arginine-rich splicing factor 2; Protein PR264; Splicing component; 35 kDa; Splicing factor SC35; SC-35; Splicing factor; arginine/serine-rich 2; SRSF2; SRSF2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of human SFRS2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 221

• Cellular Location: Nucleus. Nucleus, nucleoplasm. Nucleus speckle. Note: Phosphorylation by SRPK2 provokes its redistribution from the nuclear specke to nucleoplasm
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SRSF2 blocking peptide

Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre- mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment.

NCBI and Uniprot Product Information

• NCBI GI #: 60416437
• NCBI GeneID: 6427
• UniProt Accession #: Q01130; B3KWD5; B4DN89; H0YG49
• Molecular Weight: 24,153 Da
• NCBI Official Full Name: Serine/arginine-rich splicing factor 2
• NCBI Official Synonym Full Names: serine and arginine rich splicing factor 2
• NCBI Official Symbol: SRSF2
• NCBI Official Synonym Symbols: SC35; PR264; SC-35; SFRS2; SFRS2A; SRp30b
• NCBI Protein Information: serine/arginine-rich splicing factor 2
• UniProt Protein Name: Serine/arginine-rich splicing factor 2
• Protein Family: Serine/arginine-rich splicing factor
• UniProt Gene Name: SRSF2
• UniProt Synonym Gene Names: SFRS2; SC-35
• UniProt Entry Name: SRSF2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding the same protein and one non-coding transcript variant have been found for this gene. In addition, a pseudogene of this gene has been found on chromosome 11. [provided by RefSeq, Sep 2010]

Uniprot Description

SFRS2: Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre- mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment. Belongs to the splicing factor SR family.

Protein type: RNA splicing; Spliceosome; RNA processing

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: nuclear speck; nucleoplasm; nucleus; PML body

Molecular Function: protein binding

Biological Process: mRNA 3'-end processing; mRNA export from nucleus; mRNA processing; nuclear mRNA splicing, via spliceosome; RNA export from nucleus; RNA splicing; termination of RNA polymerase II transcription