SDS-Page
(Figure. SDS-PAGE)
Osteopontin Recombinant Protein | OPN recombinant protein
Recombinant Osteopontin (OPN)
Gene Names
Spp1; OP; 2AR; Bsp; Eta; Opn; Ric; BNSP; BSPI; Opnl; Apl-1; ETA-1; Spp-1
Applications
SDS-Page, Western Blot
Purity
>90%
Synonyms
Osteopontin; Recombinant Osteopontin (OPN); SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin; OPN recombinant protein
Host
E Coli
Purity/Purification
>90%
Form/Format
Freeze-dried powder
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Sequence Positions
Leu17~Asn294
Sequence Length
294
Applicable Applications for OPN recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Source
Prokaryotic expression
Species
Mouse
Tag
N-terminal His and GST Tag
Subcellular Location
Nucleus, Cytoplasm
Isoelectric Point
4.1
Reconstitution
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
(Figure. SDS-PAGE)
SDS-Page
(Figure. SDS-PAGE)
Product Categories/Family for OPN recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 60.7kDa
Accurate Molecular Mass: 91kDa
Accurate Molecular Mass: 91kDa
NCBI Official Full Name
Osteopontin
NCBI Official Synonym Full Names
secreted phosphoprotein 1
NCBI Official Symbol
Spp1
NCBI Official Synonym Symbols
OP; 2AR; Bsp; Eta; Opn; Ric; BNSP; BSPI; Opnl; Apl-1; ETA-1; Spp-1
NCBI Protein Information
osteopontin
UniProt Protein Name
Osteopontin
Protein Family
UniProt Gene Name
Spp1
UniProt Synonym Gene Names
Eta-1; Op; Spp-1; SPP-1
UniProt Entry Name
OSTP_MOUSE
Uniprot Description
osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space; cell projection; perinuclear region of cytoplasm; apical part of cell; cytoplasm; extracellular region
Molecular Function: extracellular matrix binding; cytokine activity
Biological Process: response to organic substance; osteoblast differentiation; neutrophil chemotaxis; ossification; response to vitamin D; response to steroid hormone stimulus; biomineral formation; positive regulation of bone resorption; cell adhesion; negative regulation of apoptosis
Research Articles on OPN
Similar Products
Product Notes
The OPN spp1 (Catalog #AAA2122724) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Leu17~Asn294. AAA Biotech's Osteopontin can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, Western Blot (WB). Researchers should empirically determine the suitability of the OPN spp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Osteopontin, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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