SPEG / APEG-1 recombinant protein

Recombinant Human SPEG / APEG-1 Protein (His tag)

Cat. Num. AAA2545035

• Gene Names: SPEG; BPEG; CNM5; APEG1; APEG-1; SPEGbeta; SPEGalpha
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: SPEG / APEG-1; Recombinant Human SPEG / APEG-1 Protein (His tag); APEG1; BPEG; KIAA1297; MGC12676; SPEGalpha; SPEGbeta; SPEG / APEG-1 recombinant protein

• Host: E Coli
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile PBS, pH 7.4
• Sequence Length: 113

• Application Notes: The recombinant human SPEG consisting of 123 amino acids and has a calculated molecular mass of 14 kDa. It migrates as an approximately 19 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for SPEG / APEG-1 recombinant protein

Background: Striated muscle preferentially expressed protein kinase, also known as aortic preferentially expressed protein 1, APEG-1, SPEG and KIAA1297, is a protein which belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. SPEG / APEG-1 contains two fibronectin type -III domains, nine Ig-like (immunoglobulin-like) domains, two protein kinase domains. Isoform 1 of SPEG is preferentially expressed in striated muscle. Non-kinase form such as isoform 3 of SPEG is predominantly expressed in the aorta. Isoform 3 of SPEG appears to be expressed only in highly differentiated ASMC in normal vessel walls and down-regulated in dedifferentiated ASMC. Isoform 3 of SPEG may have a role in regulating the growth and differentiation of arterial smooth muscle cells. Isoform 3 of SPEG is quickly down-regulated in response to vascular injury, when ASMC cells change from a quiescent to a proliferative phenotype .

Description: A DNA sequence encoding the human SPEG isoform 3 (Q15772-4) (Met 1-Glu 113) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 291042650
• NCBI GeneID: 10290
• NCBI Accession #: NP_001166947.1
• NCBI GenBank Nucleotide #: NM_001173476.1
• UniProt Accession #: Q15772; Q27J74; Q695L1; Q6FGA6; Q6ZQW1; Q6ZTL8; Q9P2P9; A8K0G6; A8MRU0
• Molecular Weight: 348,903 Da
• NCBI Official Full Name: striated muscle preferentially expressed protein kinase isoform 4
• NCBI Official Synonym Full Names: SPEG complex locus
• NCBI Official Symbol: SPEG
• NCBI Official Synonym Symbols: BPEG; CNM5; APEG1; APEG-1; SPEGbeta; SPEGalpha
• NCBI Protein Information: striated muscle preferentially expressed protein kinase
• UniProt Protein Name: Striated muscle preferentially expressed protein kinase
• UniProt Gene Name: SPEG
• UniProt Synonym Gene Names: APEG1; KIAA1297; APEG-1

NCBI Description

This gene encodes a protein with similarity to members of the myosin light chain kinase family. This protein family is required for myocyte cytoskeletal development. Along with the desmin gene, expression of this gene may be controlled by the desmin locus control region. Mutations in this gene are associated with centronuclear myopathy 5. [provided by RefSeq, Jun 2016]

Uniprot Description

SPEG: Isoform 3 may have a role in regulating the growth and differentiation of arterial smooth muscle cells. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. 4 isoforms of the human protein are produced by alternative promoter.

Protein type: CAMK group; EC 2.7.11.1; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Trio family

Chromosomal Location of Human Ortholog: 2q35

Molecular Function: protein binding

Biological Process: muscle development; negative regulation of cell proliferation

Disease: Myopathy, Centronuclear, 5