Mouse sPECAM-1 ELISA Kit | sPECAM-1/sCD31 elisa kit
Mouse sPECAM-1 (Soluble Platelet Endothelial Cell Adhesion Molecule 1) ELISA Kit
Reactivity
Mouse
Synonyms
sPECAM-1; Mouse sPECAM-1 (Soluble Platelet Endothelial Cell Adhesion Molecule 1) ELISA Kit; sPECAM-1/sCD31 elisa kit
Reactivity
Mouse
Specificity
This kit recognizes natural and recombinant Mouse sPECAM-1/sCD31. No significant cross-reactivity or interference between Mouse sPECAM-1/sCD31 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for sPECAM-1/sCD31 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse sPECAM-1/sCD31 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to sPECAM-1/sCD31. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for sPECAM-1/sCD31 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain sPECAM-1/sCD31, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of sPECAM-1/sCD31. You can calculate the concentration of sPECAM-1/sCD31 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to sPECAM-1/sCD31. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for sPECAM-1/sCD31 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain sPECAM-1/sCD31, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of sPECAM-1/sCD31. You can calculate the concentration of sPECAM-1/sCD31 in the samples by comparing the OD of the samples to the standard curve.
