Hyaluronidase PH-20 Recombinant Protein | HYALP recombinant protein

Recombinant Human Hyaluronidase PH-20

Cat. Num. AAA9420697

• Gene Names: SPAM1; HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n
• Reactivity: Human
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Hyaluronidase PH-20; Recombinant Human Hyaluronidase PH-20; Hyaluronoglucosaminidase PH-20Sperm adhesion molecule 1Sperm surface protein PH-20; HYALP recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Full Length, 36-490aa

• Tag Info: His-SUMO-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for HYALP recombinant protein

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are bedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

NCBI and Uniprot Product Information

• NCBI GI #: 291290979
• NCBI GeneID: 6677
• NCBI Accession #: NP_001167515.1
• NCBI GenBank Nucleotide #: NM_001174044.1
• UniProt Accession #: P38567; Q8TC30
• Molecular Weight: 67.83kD
• NCBI Official Full Name: hyaluronidase PH-20 isoform 2
• NCBI Official Synonym Full Names: sperm adhesion molecule 1
• NCBI Official Symbol: SPAM1
• NCBI Official Synonym Symbols: HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n
• NCBI Protein Information: hyaluronidase PH-20
• UniProt Protein Name: Hyaluronidase PH-20
• Protein Family: Hyaluronidase
• UniProt Gene Name: SPAM1
• UniProt Synonym Gene Names: HYAL3; PH20; Hyal-PH20

NCBI Description

Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

SPAM1: Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. Belongs to the glycosyl hydrolase 56 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; EC 3.2.1.35; Glycan Metabolism - glycosaminoglycan degradation; Hydrolase; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 7q31.32

Cellular Component: plasma membrane

Biological Process: binding of sperm to zona pellucida; sperm-egg recognition