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A/G-Cys Protein Recombinant Protein | A/G-Cys recombinant protein
Recombinant Protein A/G-Cys Protein (His Tag)
Purity
>90% as determined by reducing SDS-PAGE
Synonyms
A/G-Cys Protein; Recombinant Protein A/G-Cys Protein (His Tag); Protein AG; A/G-Cys recombinant protein
Host
E Coli
Purity/Purification
>90% as determined by reducing SDS-PAGE
Form/Format
Lyophilized from sterile potassium phosphate, pH7.4
Sequence
MHHHHHHHNAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDSLEG SGSGTYKLIL NGKTLKGETT TEAVDAATAE KVFKQYANDN GVDGEWTYDD ATKTFTVTEK PEVIDASELT PAVTTYKLVI NGKTLKGETT TKAVDAETAE KAFKQYANDN GVDGVWTYDD ATKTFTVTEC
Tag
N-His
Protein Construction
A DNA sequence encoding the Protein A/G was expressed with a polyhistidine tag at the N-terminus and Cys at the C-terminus.
Preparation and Storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.
This product is provided as lyophilized powder which is shipped with ice packs.
This product is provided as lyophilized powder which is shipped with ice packs.
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SDS-Page
Related Product Information for A/G-Cys recombinant protein
Protein A/G is a genetically engineered protein containing 5 IgG-binding regions of protein A and 2 of protein G. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A/G to ensure the maximum specific IgG binding. The recombinant Protein A/G is ideal for purification of polyclonal or monoclonal IgG antibodies.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,873 Da
NCBI Official Full Name
immunoglobulin G-binding protein A
UniProt Protein Name
Immunoglobulin G-binding protein A
UniProt Gene Name
spa
UniProt Synonym Gene Names
IgG-binding protein A
Uniprot Description
MiscellaneousImportant immunodiagnostic reagent because of its ability to bind the Fc fragment of a wide range of mammalian immunoglobulins.
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Product Notes
The A/G-Cys spa (Catalog #AAA2569423) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MHHHHHHHNA AQHDEAQQ NAFYQVLNMP NLNADQRN GF IQSLKD DPSQ SANV LGEAQK LN DSQAPKAD AQQNNFNKDQ QSAFYEIL NM PNLNEA QRNG FIQS LKDDPS QS TNVLGEAK KLNESQAPKA DNNFNKEQ QN AFYEIL NMPN LNEE QRNGFI QS LKDDPSQS ANLLSEAKKL NESQAPKA DN KFNKEQ QNAF YEIL HLPNLN EE QRNGFIQS LKDDPSQSAN LLAEAKKL ND AQAPKA DNKF NKEQ QNAFYE IL HLPNLTEE QRNGFIQSLK DDPSVSKE IL AEAKKL NDAQ APKE EDSLEG SG SGTYKLIL NGKTLKGETT TEAVDAAT AE KVFKQY ANDN GVDG EWTYDD AT KTFTVTEK PEVIDASELT PAVTTYKL VI NGKTLK GETT TKAV DAETAE KA FKQYANDN GVDGVWTYDD ATKTFTVT EC. It is sometimes possible for the material contained within the vial of "A/G-Cys Protein, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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