Sp100 Nuclear Recombinant Protein | Sp100 recombinant protein

Recombinant Sp100 Nuclear Antigen (Sp100)

Cat. Num. AAA2029394

• Gene Names: SP100; lysp100b
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Sp100 Nuclear; Recombinant Sp100 Nuclear Antigen (Sp100); Sp100 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 5.8 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- FERVL PNGLCFQRSD GGDPNSQLSL EQGPNGSCSQ ATLTLSPFNH SFSDGWGSND RLNSSLTHGN QSENHQLPGS PNHLGAVQLN HDLQINPCSV RLVDIKKENI SYSLDDNQQT QARTNENQGS EIIELSSDED SNDEINLSEA STLILSKPET AHSRQPPTPR TYRRGDTSDT SSSSLDRRRKRTGRMLSSSS SELSSGEESE EFLRSGSGRL GCP
• Sequence Length: 850

• Applicable Applications for Sp100 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 27.6kDa
• Accurate Molecular Mass (KD): 33kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Phe116~Pro333
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119591333
• NCBI GeneID: 6672
• Molecular Weight: 50,032 Da
• NCBI Official Full Name: SP100 nuclear antigen, isoform CRA_a
• NCBI Official Synonym Full Names: SP100 nuclear antigen
• NCBI Official Symbol: SP100
• NCBI Official Synonym Symbols: lysp100b
• NCBI Protein Information: nuclear autoantigen Sp-100
• UniProt Protein Name: Nuclear autoantigen Sp-100
• Protein Family: Nuclear autoantigen
• UniProt Gene Name: SP100
• UniProt Entry Name: SP100_HUMAN

NCBI Description

This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. [provided by RefSeq, Aug 2011]

Uniprot Description

SP100: May play a role in the control of gene expression. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: nucleoplasm; PML body; Mre11 complex; cytoplasm; nucleolus; nucleus

Molecular Function: protein domain specific binding; identical protein binding; protein binding; protein homodimerization activity; DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; transcription corepressor activity; kinase binding

Biological Process: retinoic acid receptor signaling pathway; response to retinoic acid; negative regulation of protein export from nucleus; viral reproduction; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of transcription, DNA-dependent; negative regulation of transcription factor activity; negative regulation of cell motility; negative regulation of transcription from RNA polymerase II promoter; regulation of angiogenesis; negative regulation of DNA binding; chromatin remodeling; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; negative regulation of viral transcription; response to cytokine stimulus; positive regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; telomere maintenance