SNX3 recombinant protein

SNX3, 1-162aa, Human, His tag, E Coli

Cat. Num. AAA204319

• Gene Names: SNX3; SDP3; Grd19; MCOPS8
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: SNX3; 1-162aa; Human; His tag; E Coli; Sorting nexin 3; SDP3; SNX3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAETVADTRR LITKPQNLND AYGPPSNFLE IDVSNPQTVG VGRGRFTTYE IRVKTNLPIF KLKESTVRRR YSDFEWLRSE LERESKVVVP PLPGKAFLRQ LPFRGDDGIF DDNFIEERKQ GLEQFINKVA GHPLAQNERC LHMFLQDEII DKSYTPSKIR HA
• Sequence Length: 162

• Applicable Applications for SNX3 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for SNX3 recombinant protein

Sorting nexin 3 (SNX3), also known as SDP3, is a member of the large family of hydrophilic proteins that interact with a variety of receptor types and are involved in intracellular trafficking. This protein interacts with phosphatidylinositol-3-phosphate, and is involved in protein trafficking. SNX3 comprises a distinct subgroup of nexins that share less sequence similarity outside of the PX domain and have dramatically different binding affinities for the tyrosine kinase receptors. Recombinant human SNX3 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for SNX3 recombinant protein

Signal Transduction

References

Horazdovsky B F., et al. (1997) Mol Biol Cell. 8:1529-1542.; Haft C R., et al. (1998) Mol Biol Cell. 18:7278-7287.

NCBI and Uniprot Product Information

• NCBI GI #: 4507143
• NCBI GeneID: 8724
• NCBI Accession #: NP_003786
• NCBI GenBank Nucleotide #: NM_003795.5
• UniProt Accession #: O60493; O60718; Q4TT29; Q4TT31; Q5JXJ7; Q5JXJ8; Q96AP9; Q9C0J5; Q9NU45; A8K0B1; E1P5E4; E1P5E5
• Molecular Weight: 20.9 kDa (182aa), confirmed by MALDI-TOF
• NCBI Official Full Name: sorting nexin-3 isoform a
• NCBI Official Synonym Full Names: sorting nexin 3
• NCBI Official Symbol: SNX3
• NCBI Official Synonym Symbols: SDP3; Grd19; MCOPS8
• NCBI Protein Information: sorting nexin-3
• UniProt Protein Name: Sorting nexin-3
• Protein Family: Sorting nexin
• UniProt Gene Name: SNX3

NCBI Description

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like most family members. This protein interacts with phosphatidylinositol-3-phosphate, and is involved in protein trafficking. A pseudogene of this gene is present on the sex chromosomes. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]

Uniprot Description

Phosphoinositide-binding protein required for multivesicular body formation. Specifically binds phosphatidylinositol 3-phosphate (PtdIns(P3)). Also can bind phosphatidylinositol 4-phosphate (PtdIns(P4)), phosphatidylinositol 5-phosphate (PtdIns(P5)) and phosphatidylinositol 3,5-biphosphate (PtdIns(3,5)P2) (). Plays a role in protein transport between cellular compartments. Together with RAB7A facilitates endosome membrane association of the retromer cargo-selective subcomplex (CSC/VPS). May in part act as component of the SNX3-retromer complex which mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway (PubMed:21725319, PubMed:24344282). Promotes stability and cell surface expression of epithelial sodium channel (ENAC) subunits SCNN1A and SCNN1G (). Not involved in EGFR degradation. Involved in the regulation of phagocytosis in dendritic cells possibly by regulating EEA1 recruitment to the nascent phagosomes (PubMed:23237080). Involved in iron homeostasis through regulation of endocytic recycling of the transferrin receptor TFRC presumably by delivering the transferrin:transferrin receptor complex to recycling endosomes; the function may involve the CSC retromer subcomplex (). In the case of Salmonella enterica infection plays arole in maturation of the Salmonella-containing vacuole (SCV) and promotes recruitment of LAMP1 to SCVs (PubMed:20482551).