SNRPE / Small Nuclear Ribonucleoprotein E Recombinant Protein | SNRPE recombinant protein

Human SNRPE / Small Nuclear Ribonucleoprotein E Recombinant Protein

• Gene Names: SNRPE; SME; Sm-E; HYPT11; snRNP-E
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: SNRPE / Small Nuclear Ribonucleoprotein E; Human SNRPE / Small Nuclear Ribonucleoprotein E Recombinant Protein; Small nuclear ribonucleoprotein E; snRNP-E; Sm protein E; Sm-E; SNRPE recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 92

• Applicable Applications for SNRPE recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: U1 snRNP is for instance composed of the 7 core Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least three U1 snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Component of the heptameric ring U7 snRNP complex, or U7 Sm protein core complex, at least composed of LSM10, LSM11, SNRPB, SNRPD3, SNRPE, SNRPF, SNRPG and U7 snRNA. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG; catalyzes core snRNPs assembly. Forms a 6S pICln-Sm complex composed of CLNS1A/pICln, SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG; ring-like structure where CLNS1A/pICln mimics additional Sm proteins and which is unable to assemble into the core snRNP.
• Entry Function: Core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. As part of the U7 snRNP it is involved in histone 3'-end processing. May indirectly play a role in hair development.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 4507129
• NCBI GeneID: 6635
• NCBI Accession #: NP_003085.1
• NCBI GenBank Nucleotide #: NP_003085.1
• UniProt Accession #: P62304
• NCBI Official Full Name: small nuclear ribonucleoprotein E isoform 1
• NCBI Official Synonym Full Names: small nuclear ribonucleoprotein polypeptide E
• NCBI Official Symbol: SNRPE
• NCBI Official Synonym Symbols: SME; Sm-E; HYPT11; snRNP-E
• NCBI Protein Information: small nuclear ribonucleoprotein E
• UniProt Protein Name: Small nuclear ribonucleoprotein E
• Protein Family: Small nuclear ribonucleoprotein
• UniProt Gene Name: SNRPE
• UniProt Synonym Gene Names: snRNP-E; Sm-E; SmE
• UniProt Entry Name: RUXE_HUMAN

NCBI Description

The protein encoded by this gene is a core component of U small nuclear ribonucleoproteins, which are key components of the pre-mRNA processing spliceosome. The encoded protein plays a role in the 3' end processing of histone transcripts. This protein is one of the targets in the autoimmune disease systemic lupus erythematosus, and mutations in this gene have been associated with hypotrichosis. Several pseudogenes of this gene have been identified. [provided by RefSeq, Jun 2016]

Uniprot Description

snRNP E: Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5. Belongs to the snRNP Sm proteins family.

Protein type: RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: nucleoplasm; small nuclear ribonucleoprotein complex; spliceosome; U4/U6 x U5 tri-snRNP complex; snRNP U5; snRNP U2; snRNP U1; nucleus; cytosol; U12-dependent spliceosome

Molecular Function: protein binding; RNA binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; spliceosomal snRNP biogenesis; RNA splicing; spliceosome assembly; histone mRNA metabolic process; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription; hair cycle

Disease: Hypotrichosis 11