Bovine Synuclein, Alpha ELISA Kit | SNCa elisa kit

Bovine Synuclein, Alpha ELISA Kit

Cat. Num. AAA739425

• Gene Names: SNCA; PD1; NACP; PARK1; PARK4
• Reactivity: Bovine
• Synonyms: Synuclein; Alpha; Bovine Synuclein; Alpha ELISA Kit; SNCa elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This SNCa ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine SNCa. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SNCa elisa kit

Principle of the Assay: SNCa ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SNCa antibody and an SNCa-HRP conjugate. The assay sample and buffer are incubated together with SNCa-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SNCa concentration since SNCa from samples and SNCa-HRP conjugate compete for the anti-SNCa antibody binding site. Since the number of sites is limited, as more sites are occupied by SNCa from the sample, fewer sites are left to bind SNCa-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SNCa concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SNCa elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 49456267
• NCBI GeneID: 6622
• UniProt Accession #: P37840; Q13701; Q4JHI3; Q6IAU6; A8K2A4; Q4W5L2; Q6QBS3
• Molecular Weight: 14,460 Da
• NCBI Official Full Name: SNCA
• NCBI Official Synonym Full Names: synuclein, alpha (non A4 component of amyloid precursor)
• NCBI Official Symbol: SNCA
• NCBI Official Synonym Symbols: PD1; NACP; PARK1; PARK4
• NCBI Protein Information: alpha-synuclein; synuclein alpha-140; non A-beta component of AD amyloid
• UniProt Protein Name: Alpha-synuclein
• Protein Family: Alpha-synuclein
• UniProt Gene Name: SNCA
• UniProt Synonym Gene Names: NACP; PARK1; NACP
• UniProt Entry Name: SYUA_HUMAN

NCBI Description

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

SNCA: a member of the synuclein family. Abundantly expressed in the brain. Inhibits phospholipase D2 selectively. May integrate presynaptic signaling and membrane trafficking. Implicated in the pathogenesis of Parkinson's disease. A major component of amyloid plaques in the brains of patients with Alzheimer's disease. Two alternatively spliced isoforms transcripts have been identified.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: Golgi apparatus; nuclear outer membrane; mitochondrion; rough endoplasmic reticulum; lysosome; extracellular region; fibril; terminal button; cell cortex; inclusion body; mitochondrial respiratory chain complex I; cytosol; actin cytoskeleton; synaptic vesicle; platelet alpha granule membrane; growth cone; perinuclear region of cytoplasm; axon; cytoplasm; plasma membrane; ribosome; nucleus; cell junction

Molecular Function: identical protein binding; protein domain specific binding; zinc ion binding; histone binding; kinesin binding; ferrous iron binding; microtubule binding; caspase inhibitor activity; magnesium ion binding; beta-tubulin binding; protein N-terminus binding; phosphoprotein binding; oxidoreductase activity; calcium ion binding; Hsp70 protein binding; dynein binding; protein binding; copper ion binding; phospholipase binding; phospholipid binding; tau protein binding; fatty acid binding; alpha-tubulin binding

Biological Process: regulation of long-term neuronal synaptic plasticity; negative regulation of serotonin uptake; regulation of acyl-CoA biosynthetic process; adult locomotory behavior; positive regulation of apoptosis; mitochondrial membrane organization and biogenesis; negative regulation of norepinephrine uptake; microglial cell activation; response to lipopolysaccharide; positive regulation of endocytosis; negative regulation of transcription from RNA polymerase II promoter; dopamine biosynthetic process; negative regulation of monooxygenase activity; negative regulation of caspase activity; fatty acid metabolic process; positive regulation of neurotransmitter secretion; regulation of dopamine secretion; negative regulation of histone acetylation; negative regulation of dopamine uptake; calcium ion homeostasis; negative regulation of exocytosis; response to magnesium ion; negative regulation of protein amino acid phosphorylation; behavioral response to cocaine; receptor internalization; phospholipid metabolic process; synapse organization and biogenesis; fibril organization and biogenesis; dopamine uptake; response to iron(II) ion; negative regulation of neuron apoptosis; positive regulation of receptor recycling; aging; response to drug; caspase activation; neutral lipid metabolic process; protein destabilization; negative regulation of microtubule polymerization; regulation of macrophage activation; regulation of glutamate secretion; positive regulation of peptidyl-serine phosphorylation; negative regulation of dopamine metabolic process; organelle ATP synthesis coupled electron transport; regulation of locomotion; synaptic vesicle endocytosis; positive regulation of release of sequestered calcium ion into cytosol; regulation of excitatory postsynaptic membrane potential; negative regulation of transporter activity; negative regulation of apoptosis

Disease: Parkinson Disease 4, Autosomal Dominant; Parkinson Disease 1, Autosomal Dominant; Dementia, Lewy Body