Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 Recombinant Protein | SMUG1 recombinant protein

Recombinant Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1)

Cat. Num. AAA2033573

• Gene Names: SMUG1; FDG; UNG3; HMUDG
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Single Strand Selective Monofunctional Uracil DNA Glycosylase 1; Recombinant Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1); FDG; HMUDG; UNG3; SMUG1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: MPQAFLLGSI HEPAGALMEP QPCPGSLAES FLEEELRLNA ELSQLQFSEP VGIIYNPVEY AWEPHRNYVT RYCQGPKEVL FLGMNPGPFG MAQTGVPFGE VSMVRDWLGI VGPVLTPPQE HPKRPVLGLE CPQSEVSGAR FWGFFRNLCG QPEVFFHHCF VHNLCPLLFL APSGRNLTPA ELPAKQREQL LGICDAALCR QVQLLGVRLV VGVGRLAEQR ARRALAGLMPEVQVEGLLHP SPRNPQANKG WEAVAKERLN ELGLLPLLLK
• Sequence Length: 270

• Applicable Applications for SMUG1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 33.6kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Lys270
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 344925871
• NCBI GeneID: 23583
• NCBI Accession #: NP_001230717.1
• NCBI GenBank Nucleotide #: NM_001243788.1
• Molecular Weight: 19,627 Da
• NCBI Official Full Name: single-strand selective monofunctional uracil DNA glycosylase isoform 1
• NCBI Official Synonym Full Names: single-strand-selective monofunctional uracil-DNA glycosylase 1
• NCBI Official Symbol: SMUG1
• NCBI Official Synonym Symbols: FDG; UNG3; HMUDG
• NCBI Protein Information: single-strand selective monofunctional uracil DNA glycosylase
• UniProt Protein Name: Single-strand selective monofunctional uracil DNA glycosylase
• Protein Family: Single-strand selective monofunctional uracil DNA glycosylase
• UniProt Gene Name: SMUG1
• UniProt Entry Name: SMUG1_HUMAN

NCBI Description

This gene encodes a protein that participates in base excision repair by removing uracil from single- and double-stranded DNA. Many alternatively spliced transcript variants exist for this gene; the full-length nature is known for some but not all of the variants. [provided by RefSeq, Aug 2011]

Uniprot Description

SMUG1: Responsible for recognizing base lesions in the genome and initiating base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA and has a preference for single-stranded DNA substrates. The activity is greater against mismatches (U/G) than against matches (U/A). Excised uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5- hydroxymethyluracil (hmU)] in ssDNA and dsDNA but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine) and other oxidized damage. The activity is damage specificity and salt concentration-dependent. The general order of the preference for ssDNA and dsDNA is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at the low salt concentration. At the high concentration dsDNA (G pair) > dsDNA (A pair) > ssDNA. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.2.2.-; DNA repair, damage

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: nucleolus; nucleoplasm

Molecular Function: DNA binding; DNA N-glycosylase activity; oxidized pyrimidine base lesion DNA N-glycosylase activity; protein binding; single-strand selective uracil DNA N-glycosylase activity; uracil DNA N-glycosylase activity

Biological Process: base-excision repair; base-excision repair, AP site formation; depyrimidination; DNA repair