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Typical Testing Data/Standard Curve (for reference only)

Human sphingomyelin phosphodiesterase 1, acid lysosomal ELISA Kit | SMPD1 elisa kit

Human acid sphingomyelinase, ASM ELISA Kit

Gene Names
SMPD1; ASM; NPD; ASMASE
Reactivity
Human
Synonyms
sphingomyelin phosphodiesterase 1; acid lysosomal; Human acid sphingomyelinase; ASM ELISA Kit; ASM; NPD; acid sphingomyelinase; SMPD1 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human ASM. No significant cross-reactivity or interference between human ASM and analogues was observed.
Sequence Length
631
Samples
Serum, plasma, cell culture supernates, cerebrospinal fluid (csf) and urine
Assay Type
Quantitative Sandwich
Detection Range
1.25 ng/ml-80 ng/ml
Sensitivity
< 0.31 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SMPD1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ASM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ASM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ASM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ASM bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
69,752 Da
NCBI Official Full Name
sphingomyelin phosphodiesterase isoform 1
NCBI Official Synonym Full Names
sphingomyelin phosphodiesterase 1, acid lysosomal
NCBI Official Symbol
SMPD1
NCBI Official Synonym Symbols
ASM; NPD; ASMASE
NCBI Protein Information
sphingomyelin phosphodiesterase; acid sphingomyelinase
UniProt Protein Name
Sphingomyelin phosphodiesterase
UniProt Gene Name
SMPD1
UniProt Synonym Gene Names
ASM; aSMase
UniProt Entry Name
ASM_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]

Uniprot Description

Function: Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity.

Catalytic activity: Sphingomyelin + H2O = N-acylsphingosine + phosphocholine.

Research Articles on SMPD1

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Product Notes

The Human SMPD1 smpd1 (Catalog #AAA700800) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA700800 ELISA Kit recognizes Human SMPD1. It is sometimes possible for the material contained within the vial of "sphingomyelin phosphodiesterase 1, acid lysosomal, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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