Mothers Against Decapentaplegic Homolog 5 Recombinant Protein | Smad5 recombinant protein

Recombinant Mothers Against Decapentaplegic Homolog 5 (Smad5)

Cat. Num. AAA2029450

• Gene Names: SMAD5; DWFC; JV5-1; MADH5
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Mothers Against Decapentaplegic Homolog 5; Recombinant Mothers Against Decapentaplegic Homolog 5 (Smad5); Smad5 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 6.4 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- G PGSPFQLPAD TPPPAYMPPD DQMGQDNSQP MDTSNNMIPQ IMPSISSRDV QPVAYEEPKH WCSIVYYELN NRVGEAFHAS STSVLVDGFT DPSNNKSRFC LGLLSNVNRN STIENTRRHI GKGVHLYYVG GEVYAECLSD SSIFVQSRNC NFHHGFHPTT VCKIPSSCSL KIFNNQEFAQ LLAQSVNHGF EAVYELTKMCTIRMSFVKGW GAEYHRQDVT STPCWIEIHL HGPLQWLDKV LTQMGSPLNP ISSV
• Sequence Length: 465

• Applicable Applications for Smad5 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 32.3kDa
• Accurate Molecular Mass (KD): 32kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Gly210~Val464
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 47778931
• NCBI GeneID: 4090
• NCBI Accession #: NP_001001420.1
• NCBI GenBank Nucleotide #: NM_001001420.2
• Molecular Weight: 52,258 Da
• NCBI Official Full Name: mothers against decapentaplegic homolog 5
• NCBI Official Synonym Full Names: SMAD family member 5
• NCBI Official Symbol: SMAD5
• NCBI Official Synonym Symbols: DWFC; JV5-1; MADH5
• NCBI Protein Information: mothers against decapentaplegic homolog 5
• UniProt Protein Name: Mothers against decapentaplegic homolog 5
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: SMAD5
• UniProt Synonym Gene Names: MADH5; MAD homolog 5; Mothers against DPP homolog 5; SMAD 5; Smad5; hSmad5
• UniProt Entry Name: SMAD5_HUMAN

NCBI Description

The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

SMAD5: transcription factor phosphorylated and activated by bone morphogenetic protein receptor kinases. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where they regulate transcription of target genes. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; transcription factor complex; protein complex; cytoplasm; integral to membrane; intracellular; cytosol; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding; metal ion binding; receptor signaling protein activity; transcription factor activity; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity

Biological Process: Mullerian duct regression; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; signal transduction; embryonic pattern specification; protein amino acid phosphorylation; germ cell development; BMP signaling pathway; positive regulation of osteoblast differentiation; ureteric bud development; cartilage development; transforming growth factor beta receptor signaling pathway; erythrocyte differentiation; osteoblast fate commitment; cardiac muscle contraction