Rat Mothers Against Decapentaplegic Homolog 4 ELISA Kit | Smad4 elisa kit

Rat Mothers Against Decapentaplegic Homolog 4 ELISA Kit

Cat. Num. AAA764485

• Gene Names: SMAD4; JIP; DPC4; MADH4; MYHRS
• Reactivity: Rat
• Synonyms: Mothers Against Decapentaplegic Homolog 4; Rat Mothers Against Decapentaplegic Homolog 4 ELISA Kit; Smad4/MADH4/MAD homolog 4/Mothers against DPP homolog 4/Deletion target in pancreatic carcinoma 4/SMAD family member 4/SMAD 4/Smad4/Hsmad4; Smad4 elisa kit

• Reactivity: Rat
• Sequence Length: 552

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Smad4 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Smad4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Smad4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Smad4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Smad4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4885457
• NCBI GeneID: 4089
• NCBI Accession #: NP_005350.1
• NCBI GenBank Nucleotide #: NM_005359.5
• Molecular Weight: 60,439 Da
• NCBI Official Full Name: mothers against decapentaplegic homolog 4
• NCBI Official Synonym Full Names: SMAD family member 4
• NCBI Official Symbol: SMAD4
• NCBI Official Synonym Symbols: JIP; DPC4; MADH4; MYHRS
• NCBI Protein Information: mothers against decapentaplegic homolog 4
• UniProt Protein Name: Mothers against decapentaplegic homolog 4
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: SMAD4
• UniProt Synonym Gene Names: DPC4; MADH4; MAD homolog 4; Mothers against DPP homolog 4; SMAD 4; Smad4; hSMAD4
• UniProt Entry Name: SMAD4_HUMAN

NCBI Description

This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Oct 2009]

Uniprot Description

SMAD4: transcription factor that mediates signal transduction by the transforming growth factor superfamily. The common smad (co-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. Promotes binding of the Smad2/Smad4/Fast-1 complex to DNA and provides an activation function required for Smad1 or Smad2 to stimulate transcription.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin binding; collagen binding; DNA binding; identical protein binding; metal ion binding; protein binding; protein heterodimerization activity; protein homodimerization activity; sequence-specific DNA binding; transcription factor activity; transforming growth factor beta receptor, common-partner cytoplasmic mediator activity

Biological Process: axon guidance; BMP signaling pathway; cell proliferation; cellular iron ion homeostasis; developmental growth; embryonic digit morphogenesis; endoderm development; endothelial cell activation; formation of anatomical boundary; gastrulation with mouth forming second; gene expression; in utero embryonic development; mesoderm development; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of protein catabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neural crest cell differentiation; neuron fate commitment; palate development; positive regulation of BMP signaling pathway; positive regulation of histone H3-K4 methylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of binding; regulation of hair follicle development; regulation of transforming growth factor beta receptor signaling pathway; regulation of transforming growth factor-beta2 production; response to hypoxia; sebaceous gland development; SMAD protein complex assembly; somatic stem cell maintenance; somite rostral/caudal axis specification; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; ureteric bud branching

Disease: Juvenile Polyposis Syndrome; Juvenile Polyposis/hereditary Hemorrhagic Telangiectasia Syndrome; Myhre Syndrome; Pancreatic Cancer