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SDS-Page (Figure. SDS-PAGE)

Secretory Leukocyte Peptidase Inhibitor Recombinant Protein | SLPI recombinant protein

Recombinant Secretory Leukocyte Peptidase Inhibitor (SLPI)

Gene Names
SLPI; ALP; MPI; ALK1; BLPI; HUSI; WAP4; WFDC4; HUSI-I
Applications
SDS-Page, Western Blot
Purity
>90%
Synonyms
Secretory Leukocyte Peptidase Inhibitor; Recombinant Secretory Leukocyte Peptidase Inhibitor (SLPI); ALK1; ALP; MPI; BLPI; HUSI; HUSI-I; WAP4; WFDC4; Antileukoproteinase; Seminal proteinase inhibitor; Mucus proteinase inhibitor; WAP four-disulfide core domain protein 4; SLPI recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
>90%
Form/Format
Freeze-dried powder
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Sequence Positions
Ser26~Ala132
Sequence Length
132
Applicable Applications for SLPI recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Source
Prokaryotic expression
Species
Human
Tag
N-terminal His Tag
Subcellular Location
Secreted
Isoelectric Point
9.1
Reconstitution
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

(Figure. SDS-PAGE)

SDS-Page (Figure. SDS-PAGE)
Product Categories/Family for SLPI recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 15.4kDa
Accurate Molecular Mass: 15kDa
NCBI Official Full Name
secretory leukocyte protease inhibitor (SLPI)
NCBI Official Synonym Full Names
secretory leukocyte peptidase inhibitor
NCBI Official Symbol
SLPI
NCBI Official Synonym Symbols
ALP; MPI; ALK1; BLPI; HUSI; WAP4; WFDC4; HUSI-I
NCBI Protein Information
antileukoproteinase
UniProt Protein Name
Antileukoproteinase
Protein Family
UniProt Gene Name
SLPI
UniProt Synonym Gene Names
WAP4; WFDC4; ALP; MPI
UniProt Entry Name
SLPI_HUMAN

NCBI Description

This gene encodes a secreted inhibitor which protects epithelial tissues from serine proteases. It is found in various secretions including seminal plasma, cervical mucus, and bronchial secretions, and has affinity for trypsin, leukocyte elastase, and cathepsin G. Its inhibitory effect contributes to the immune response by protecting epithelial surfaces from attack by endogenous proteolytic enzymes. This antimicrobial protein has antibacterial, antifungal and antiviral activity. [provided by RefSeq, Nov 2014]

Uniprot Description

SLPI: Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues.

Protein type: Secreted; Inhibitor; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: extracellular matrix

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; endopeptidase inhibitor activity

Biological Process: negative regulation of viral genome replication; negative regulation of protein binding

Research Articles on SLPI

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Product Notes

The SLPI slpi (Catalog #AAA2122806) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Ser26~Ala132. AAA Biotech's Secretory Leukocyte Peptidase Inhibitor can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, Western Blot (WB). Researchers should empirically determine the suitability of the SLPI slpi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Secretory Leukocyte Peptidase Inhibitor, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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