Rat Solute carrier organic anion transporter family member 1A1 ELISA Kit | Slco1a1 elisa kit

Rat Solute carrier organic anion transporter family member 1A1 ELISA Kit

Cat. Num. AAA288254

• Gene Names: Slco1a1; Oatp1; OATP-1; Slc21a1; Slc21a3
• Reactivity: Rat
• Synonyms: Solute carrier organic anion transporter family member 1A1; Rat Solute carrier organic anion transporter family member 1A1 ELISA Kit; Slco1a1 elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural Rat Slco1a1. No significant cross-reactivity or interference was observed.
• Sequence Length: 670

• Assay Type: Competitive
• Detection Range: 0.625-40 ng/mL
• Sensitivity: 0.312 ng/mL
• Intra-Assay CV: <=4.7%
• Inter-Assay CV: <=8.5%
• Recovery: 86%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Slco1a1 elisa kit

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Slco1a1, During the reaction, Slco1a1 in the sample or standard competes with a fixed amount of biotin-labeled Slco1a1 for sites on a pre-coated Monoclonal antibody specific to Slco1a1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Slco1a1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8394294
• NCBI GeneID: 50572
• NCBI Accession #: NP_058807.1
• NCBI GenBank Nucleotide #: NM_017111.1
• UniProt Accession #: P46720
• Molecular Weight: 74,178 Da
• NCBI Official Full Name: solute carrier organic anion transporter family member 1A1
• NCBI Official Synonym Full Names: solute carrier organic anion transporter family, member 1a1
• NCBI Official Symbol: Slco1a1
• NCBI Official Synonym Symbols: Oatp1; OATP-1; Slc21a1; Slc21a3
• NCBI Protein Information: solute carrier organic anion transporter family member 1A1
• UniProt Protein Name: Solute carrier organic anion transporter family member 1A1
• Protein Family: Solute carrier organic anion transporter family
• UniProt Gene Name: Slco1a1
• UniProt Synonym Gene Names: Oatp1; Oatp1a1; Slc21a1; OATP-1
• UniProt Entry Name: SO1A1_RAT

NCBI Description

mediates the transport of sulfated, amidated bile acid, sulfolithocholyltaurine, into rat hepatocytes [RGD, Feb 2006]

Uniprot Description

SLCO1A1: Mediates the Na(+)-independent transport of organic anions such as taurocholate, prostaglandin E2 (PGE2), dehydroepiandrosterone sulfate (DHEAS), 17-beta-glucuronosyl estradiol, estrone-3-sulfate, sulfobromophthalein (BSP), ouabain and gadoxetate. Belongs to the organo anion transporter (TC 2.A.60) family.

Protein type: Membrane protein, integral; Transporter

Cellular Component: basolateral plasma membrane; integral to plasma membrane

Molecular Function: bile acid transmembrane transporter activity; organic anion transmembrane transporter activity; sodium-independent organic anion transmembrane transporter activity

Biological Process: bile acid and bile salt transport; bile acid metabolic process; organic anion transport; response to testosterone stimulus; sodium-independent organic anion transport