Organic Cation/Ergothioneine Transporter Recombinant Protein | OCTN1 recombinant protein

Recombinant Organic Cation/Ergothioneine Transporter (OCTN1)

Cat. Num. AAA2030285

• Gene Names: SLC22A4; OCTN1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Organic Cation/Ergothioneine Transporter; Recombinant Organic Cation/Ergothioneine Transporter (OCTN1); OCTN1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; LAGTPEHRC RVPDAANLSS AWRNNSVPLR LRDGREVPHS CSRYRLATIA NFSALGLEPGRDVDLGQLEQ ESCLDGWEFS QDVYLSTVVT EWNLVCEDNW K
• Sequence Length: 551

• Applicable Applications for OCTN1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 15.0kDa
• Accurate Molecular Mass (KD): 18kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Leu42~Lys141
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 2605501
• NCBI GeneID: 6583
• Molecular Weight: 62,155 Da
• NCBI Official Full Name: OCTN1
• NCBI Official Synonym Full Names: solute carrier family 22 (organic cation/zwitterion transporter), member 4
• NCBI Official Symbol: SLC22A4
• NCBI Official Synonym Symbols: OCTN1
• NCBI Protein Information: solute carrier family 22 member 4
• UniProt Protein Name: Solute carrier family 22 member 4
• UniProt Gene Name: SLC22A4
• UniProt Synonym Gene Names: ETT; OCTN1; UT2H; ET transporter
• UniProt Entry Name: S22A4_HUMAN

NCBI Description

Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. The encoded protein is an organic cation transporter and plasma integral membrane protein containing eleven putative transmembrane domains as well as a nucleotide-binding site motif. Transport by this protein is at least partially ATP-dependent. [provided by RefSeq, Jul 2008]

Uniprot Description

SLC22A4: Sodium-ion dependent, low affinity carnitine transporter. Probably transports one sodium ion with one molecule of carnitine. Also transports organic cations such as tetraethylammonium (TEA) without the involvement of sodium. Relative uptake activity ratio of carnitine to TEA is 1.78. A key substrate of this transporter seems to be ergothioneine (ET). Genetic variations in SLC22A4 are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family; Transporter; Mitochondrial

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: mitochondrion; integral to plasma membrane; apical plasma membrane; plasma membrane

Molecular Function: protein binding; cation:cation antiporter activity; symporter activity; carnitine transporter activity; nucleotide binding; secondary active organic cation transmembrane transporter activity; quaternary ammonium group transmembrane transporter activity; ATP binding; PDZ domain binding

Biological Process: quaternary ammonium group transport; organic cation transport; triacylglycerol metabolic process; sodium ion transport; carnitine metabolic process; body fluid secretion; transmembrane transport; carnitine transport

Disease: Rheumatoid Arthritis