Excitatory Amino Acid Transporter 4 Recombinant Protein | EAAT4 recombinant protein

Recombinant Excitatory Amino Acid Transporter 4 (EAAT4)

Cat. Num. AAA2033191

• Gene Names: SLC1A6; EAAT4
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Excitatory Amino Acid Transporter 4; Recombinant Excitatory Amino Acid Transporter 4 (EAAT4); SLC1A6; Solute Carrier Family 1 Member 6; High Affinity Aspartate/Glutamate Transporter; Sodium-dependent glutamate/aspartate transporter; EAAT4 recombinant protein

• Host: E Coli
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: GKGSK EGLHREGRIE TIPTADAFMD LIRNMFPPNL VEACFKQFKT QYSTRVVTRTMVRTENGSEP GASMPPPFSV ENGTSFLENV TRALGTLQEM LSFEETVPVP GSAN
• Sequence Length: 312

• Applicable Applications for EAAT4 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 15.7kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Gly156~Asn264
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 442535523
• NCBI GeneID: 6511
• NCBI Accession #: NP_001259017.1
• NCBI GenBank Nucleotide #: NM_001272088.1
• Molecular Weight: 34,846 Da
• NCBI Official Full Name: excitatory amino acid transporter 4 isoform 1
• NCBI Official Synonym Full Names: solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6
• NCBI Official Symbol: SLC1A6
• NCBI Official Synonym Symbols: EAAT4
• NCBI Protein Information: excitatory amino acid transporter 4
• UniProt Protein Name: Excitatory amino acid transporter 4
• Protein Family: Excitatory amino acid transporter
• UniProt Gene Name: SLC1A6
• UniProt Synonym Gene Names: EAAT4
• UniProt Entry Name: EAA4_HUMAN

Uniprot Description

SLC1A6: Transports L-glutamate and also L- and D-aspartate. Seems to act as a symport by cotransporting sodium. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A6 subfamily.

Protein type: Transporter, SLC family; Membrane protein, multi-pass; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: Golgi apparatus; integral to plasma membrane; membrane; plasma membrane

Molecular Function: L-aspartate transmembrane transporter activity; L-glutamate transmembrane transporter activity; sodium:dicarboxylate symporter activity

Biological Process: aspartate transport; glutamate secretion; ion transport; L-glutamate transport; neurotransmitter secretion; regulation of membrane potential; synaptic transmission; transmembrane transport