Typical Testing Data/Standard Curve (for reference only)
Canine Anti-soluble liver antigen ELISA Kit | SLA elisa kit
Canine Anti-soluble liver antigen ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: SLA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SLA antibody and an SLA-HRP conjugate. The assay sample and buffer are incubated together with SLA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLA concentration since SLA from samples and SLA-HRP conjugate compete for the anti-SLA antibody binding site. Since the number of sites is limited, as more sites are occupied by SLA from the sample, fewer sites are left to bind SLA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLA concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The amino acid selenocysteine is the only amino acid that does not have its own tRNA synthetase. Instead, this amino acid is synthesized on its cognate tRNA in a three step process. The protein encoded by this gene catalyzes the third step in the process, the conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec).[provided by RefSeq, Mar 2011]
Uniprot Description
SLA/LP: Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl- tRNA(Sec) required for selenoprotein biosynthesis. Defects in SEPSECS are the cause of pontocerebellar hypoplasia type 2D (PCH2D). PCH2D is a disorder characterized by postnatal onset of progressive atrophy of the cerebrum and cerebellum, microcephaly, profound mental retardation, spasticity, and variable seizures. Belongs to the SepSecS family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding; Transferase; EC 2.9.1.2
Chromosomal Location of Human Ortholog: 4p15.2
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding; transferase activity, transferring selenium-containing groups; tRNA binding; pyridoxal phosphate binding
Biological Process: selenocysteine incorporation
Disease: Pontocerebellar Hypoplasia, Type 2d
Research Articles on SLA
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Product Notes
The Canine SLA sepsecs (Catalog #AAA741956) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA741956 ELISA Kit recognizes Canine SLA. It is sometimes possible for the material contained within the vial of "Anti-soluble liver antigen, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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