NAD-dependent protein deacetylase sirtuin-4 (Sirt4) Recombinant Protein | Sirt4 recombinant protein

Recombinant Mouse NAD-dependent protein deacetylase sirtuin-4 (Sirt4)

Cat. Num. AAA1363193

• Gene Names: Sirt4; 4930596O17Rik
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: NAD-dependent protein deacetylase sirtuin-4 (Sirt4); Recombinant Mouse NAD-dependent protein deacetylase sirtuin-4 (Sirt4); Sirt4 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 26-333, full length protein
• Sequence: SGLFVPPSPPLDPEKIKELQRFISLSKKLLVMTGAGISTESGIPDYRSEKVGLYARTDRRPIQHIDFVRSAPVRQRYWARNFVGWPQFSSHQPNPAHWALSNWERLGKLHWLVTQNVDALHSKAGSQRLTELHGCMHRVLCLNCGEQTARRVLQERFQALNPSWSAEAQGVAPDGDVFLTEEQVRSFQVPCCDRCGGPLKPDVVFFGDTVNPDKVDFVHRRVKEADSLLVVGSSLQVYSGYRFILTAREQKLPIAILNIGPTRSDDLACLKLDSRCGELLPLIDPRRQHSDVQRLEMNFPLSSAAQDP
• Sequence Length: 308

• Species: Mouse
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for Sirt4 recombinant protein

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. This protein is included in class IV of the sirtuin family.

NCBI and Uniprot Product Information

• NCBI GI #: 267844849
• NCBI GeneID: 75387
• NCBI Accession #: NP_001161163.1
• NCBI GenBank Nucleotide #: NM_001167691.1
• UniProt Accession #: Q8R216; D3Z1D9
• Molecular Weight: 37,525 Da
• NCBI Official Full Name: NAD-dependent protein lipoamidase sirtuin-4, mitochondrial
• NCBI Official Synonym Full Names: sirtuin 4
• NCBI Official Symbol: Sirt4
• NCBI Official Synonym Symbols: 4930596O17Rik
• NCBI Protein Information: NAD-dependent protein lipoamidase sirtuin-4, mitochondrial
• UniProt Protein Name: NAD-dependent protein lipoamidase sirtuin-4, mitochondrial
• Protein Family: NAD-dependent protein deacylase
• UniProt Gene Name: Sirt4
• UniProt Synonym Gene Names: Sir2l4

Uniprot Description

Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase (PubMed:19220062). Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner (PubMed:25525879). Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest (PubMed:16959573). In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation (PubMed:23663782). Acts as a tumor suppressor (PubMed:23562301, PubMed:23663782). Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis (PubMed:23746352). Does not seem to deacetylate PC (PubMed:23438705). Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1:PPARA interaction probably through the regulation of NAD+ levels (PubMed:24043310, PubMed:20685656). Down-regulates insulin secretion ().