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Glycated Hemoglobin A1c (HbA1c) Native Protein | HbA1c native protein

Native Glycated Hemoglobin A1c (HbA1c)

Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%. Affinity Chromatography
Synonyms
Glycated Hemoglobin A1c (HbA1c); Native Glycated Hemoglobin A1c (HbA1c); HbA1c native protein
Ordering
For Research Use Only!
Host
Simian
Purity/Purification
> 95%. Affinity Chromatography
Form/Format
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Sequence
The HbA1c shows the average amount of glucose in the blood over a period of 3 months. Sugar in the bloodstream can become attached to the hemoglobin in red blood cells (glycosylation). Once the sugar is attached, it stays there for the life of the red blood cell, which is about 120 days. The higher the level of blood sugar, the more sugar attaches to red blood cells. The HbA1c is formed in a non-enzymatic pathway by hemoglobin's standard exposure to elevated plasma levels of glucose. HbA1c is tested to monitor nephropathy and retinopathy in diabetes mellitus.
Applicable Applications for HbA1c native protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Organism
Rhesus monkey (Simian)
Antibody Specificity
The antibody is a mouse monoclonal antibody raised against HbA1c conjugated to simian. It has beenselected for its ability to recognize HbA1c in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezerfor one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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Product Notes

The HbA1c (Catalog #AAA2018521) is a Native Protein produced from Simian and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Glycated Hemoglobin A1c (HbA1c) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the HbA1c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The HbA1c shows the average amount of glucose in the blood over a period of 3 months. Sugar in the bloodstrea m can become attached to the hemoglobin in red blood cells (glycosyla tion). Once the sugar is attached, it stays there for the life of the red blood cell, which is about 120 days. The higher the level of blood sugar, the more sugar attaches to red blood cells. The HbA1c is formed in a non-enzyma tic pathway by hemoglobin 's standard exposure to elevated plasma levels of glucose. HbA1c is tested to monitor nephropath y and retinopath y in diabetes mellitus. It is sometimes possible for the material contained within the vial of "Glycated Hemoglobin A1c (HbA1c), Native Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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