Typical Testing Data/Standard Curve (for reference only)
Sheep tPA ELISA Kit | tPA elisa kit
Sheep tPA (Plasminogen Activator, Tissue) ELISA Kit
Gene Names
PLAT; TPA; T-PA
Reactivity
Sheep
Synonyms
tPA; Sheep tPA (Plasminogen Activator; Tissue) ELISA Kit; tPA elisa kit
Reactivity
Sheep
Specificity
This kit recognizes natural and recombinant Sheep tPA. No significant cross-reactivity or interference between Sheep tPA and analogues was observed.
Sequence Length
19
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for tPA elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep tPA concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to tPA. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for tPA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain tPA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of tPA. You can calculate the concentration of tPA in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to tPA. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for tPA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain tPA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of tPA. You can calculate the concentration of tPA in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
2,342 Da
NCBI Official Full Name
tPA, partial
NCBI Official Synonym Full Names
plasminogen activator, tissue
NCBI Official Symbol
PLAT
NCBI Official Synonym Symbols
TPA; T-PA
NCBI Protein Information
tissue-type plasminogen activator; alteplase; plasminogen activator, tissue type; reteplase; t-plasminogen activator; tissue plasminogen activator (t-PA)
UniProt Protein Name
TPA protein
Protein Family
UniProt Gene Name
tPA
UniProt Entry Name
Q6LBF5_HUMAN
NCBI Description
This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Research Articles on tPA
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Product Notes
The Sheep tPA tpa (Catalog #AAA2503889) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2503889 ELISA Kit recognizes Sheep tPA. It is sometimes possible for the material contained within the vial of "tPA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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