Sheep S100A7 ELISA Kit | S100A7 elisa kit

Sheep S100A7 (S100 Calcium Binding Protein A7) ELISA Kit

Cat. Num. AAA2500953

• Reactivity: Sheep
• Synonyms: S100A7; Sheep S100A7 (S100 Calcium Binding Protein A7) ELISA Kit; S100A7 elisa kit

• Reactivity: Sheep
• Specificity: This kit recognizes natural and recombinant Sheep S100A7. No significant cross-reactivity or interference between Sheep S100A7 and analogues was observed.
• Sequence Length: 101

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100A7 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep S100A7 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to S100A7. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for S100A7 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain S100A7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of S100A7. You can calculate the concentration of S100A7 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 49457324
• UniProt Accession #: P31151; Q5SY67; Q6FGE3; Q9H1E2
• Molecular Weight: 11,471 Da
• NCBI Official Full Name: S100A7
• UniProt Protein Name: Protein S100-A7
• Protein Family: Protein
• UniProt Gene Name: S100A7
• UniProt Synonym Gene Names: PSOR1; S100A7C
• UniProt Entry Name: S10A7_HUMAN

Uniprot Description

S100A7: is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein differs from the other S100 proteins of known structure in its lack of calcium binding ability in one EF-hand at the N-terminus. This protein is markedly over-expressed in the skin lesions of psoriatic patients, but is excluded as a candidate gene for familial psoriasis susceptibility. The exact function of this protein is not known. [provided by RefSeq, Jul 2008]

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: focal adhesion; endoplasmic reticulum; cytoplasm; extracellular region; cytosol; nucleus

Molecular Function: RAGE receptor binding; protein binding; zinc ion binding; calcium ion binding

Biological Process: keratinocyte differentiation; sequestering of metal ion; epidermis development; response to reactive oxygen species; innate immune response; response to lipopolysaccharide; angiogenesis; defense response to Gram-negative bacterium