Sheep Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit | MED12 elisa kit

Sheep Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit

Cat. Num. AAA7247540

• Gene Names: MED12; OKS; FGS1; HOPA; OPA1; OHDOX; ARC240; CAGH45; MED12S; TNRC11; TRAP230
• Reactivity: Sheep
• Synonyms: Mediator of RNA polymerase II transcription subunit 12 (MED12); Sheep Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit; MED12 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Preparation and Storage: Store all reagents at 2-8 degree C.

Product Categories/Family for MED12 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 110347429
• NCBI GeneID: 9968
• NCBI Accession #: NP_005111.2
• NCBI GenBank Nucleotide #: NM_005120.2
• Molecular Weight: 242,953 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 12
• NCBI Official Synonym Full Names: mediator complex subunit 12
• NCBI Official Symbol: MED12
• NCBI Official Synonym Symbols: OKS; FGS1; HOPA; OPA1; OHDOX; ARC240; CAGH45; MED12S; TNRC11; TRAP230
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 12; CAG repeat protein 45; human opposite paired; OPA-containing protein; putative mediator subunit 12; activator-recruited cofactor 240 kDa component; trinucleotide repeat-containing gene 11 protein; thyroid hormone receptor-associated protein, 230 kDa subunit; mediator of RNA polymerase II transcription, subunit 12 homolog; thyroid hormone receptor-associated protein complex 230 kDa component; trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit)
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 12
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED12
• UniProt Synonym Gene Names: ARC240; CAGH45; HOPA; KIAA0192; TNRC11; TRAP230; ARC240; Trap230
• UniProt Entry Name: MED12_HUMAN

NCBI Description

The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009]

Uniprot Description

MED12: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Also interacts with CTNNB1 and GLI3. Ubiquitous. Belongs to the Mediator complex subunit 12 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: nucleoplasm; centrosome; membrane; nucleolus; Srb-mediator complex; nucleus

Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; vitamin D receptor binding; transcription coactivator activity; beta-catenin binding; transcription cofactor activity; thyroid hormone receptor binding; receptor activity; chromatin binding

Biological Process: steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; Schwann cell development; negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway; heart development; positive regulation of transcription, DNA-dependent; neural tube closure; androgen receptor signaling pathway; stem cell maintenance; positive regulation of transcription from RNA polymerase II promoter; gene expression; Wnt receptor signaling pathway through beta-catenin; oligodendrocyte development

Disease: Ohdo Syndrome, X-linked; Opitz-kaveggia Syndrome; Lujan-fryns Syndrome