Typical Testing Data/Standard Curve (for reference only)
Sheep gamma-ECS ELISA Kit | gamma-ECS elisa kit
Sheep gamma-ECS (gamma-Glutamyl Systeine Synthetase) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to gamma-ECS. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for gamma-ECS and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain gamma-ECS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of gamma-ECS. You can calculate the concentration of gamma-ECS in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]
Uniprot Description
GCLC: Defects in GCLC are the cause of hemolytic anemia (HAGGSD). Belongs to the glutamate--cysteine ligase type 3 family.
Protein type: Ligase; EC 6.3.2.2; Other Amino Acids Metabolism - glutathione
Chromosomal Location of Human Ortholog: 6p12
Cellular Component: glutamate-cysteine ligase complex; cytosol
Molecular Function: glutamate binding; protein heterodimerization activity; glutamate-cysteine ligase activity; magnesium ion binding; ADP binding; coenzyme binding; ATP binding
Biological Process: glutamate metabolic process; response to arsenic; L-ascorbic acid metabolic process; response to hormone stimulus; response to nitrosative stress; apoptotic mitochondrial changes; cysteine metabolic process; cell redox homeostasis; sulfur amino acid metabolic process; response to heat; xenobiotic metabolic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; glutathione biosynthetic process; negative regulation of protein ubiquitination; response to oxidative stress; negative regulation of neuron apoptosis; regulation of mitochondrial depolarization; regulation of blood vessel size; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
Disease: Gamma-glutamylcysteine Synthetase Deficiency, Hemolytic Anemia Due To; Myocardial Infarction, Susceptibility To
Research Articles on gamma-ECS
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Product Notes
The Sheep gamma-ECS gclc (Catalog #AAA2500367) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2500367 ELISA Kit recognizes Sheep gamma-ECS. It is sometimes possible for the material contained within the vial of "gamma-ECS, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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