Typical Testing Data/Standard Curve (for reference only)
Sheep Cytochrome P450 3A7 (CYP3A7) ELISA Kit | CYP3A7 elisa kit
Sheep Cytochrome P450 3A7 (CYP3A7) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CYP3A4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CYP3A4 antibody and an CYP3A4-HRP conjugate. The assay sample and buffer are incubated together with CYP3A4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYP3A4 concentration since CYP3A4 from samples and CYP3A4-HRP conjugate compete for the anti-CYP3A4 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYP3A4 from the sample, fewer sites are left to bind CYP3A4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP3A4 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cytochrome P450 superfamily of enzymes, which participate in drug metabolism and the synthesis of cholesterol, steroids and other lipids. This enzyme hydroxylates testosterone and dehydroepiandrosterone 3-sulphate, which is involved in the formation of estriol during pregnancy. This gene is part of a cluster of related genes on chromosome 7q21.1. Naturally-occurring readthrough transcription occurs between this gene and the downstream CYP3AP1 pseudogene and is represented by GeneID:100861540. [provided by RefSeq, Apr 2014]
Uniprot Description
CYP3A7: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Belongs to the cytochrome P450 family.
Protein type: Oxidoreductase; Xenobiotic Metabolism - drug metabolism - other enzymes; Lipid Metabolism - linoleic acid; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.14.14.1; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - metabolism by cytochrome P450
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: endoplasmic reticulum membrane
Molecular Function: iron ion binding; heme binding; oxygen binding; monooxygenase activity
Biological Process: xenobiotic metabolic process
Research Articles on CYP3A7
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Product Notes
The Sheep CYP3A7 cyp3a7 (Catalog #AAA7228875) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7228875 ELISA Kit recognizes Sheep CYP3A7. It is sometimes possible for the material contained within the vial of "Cytochrome P450 3A7 (CYP3A7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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