Typical Testing Data/Standard Curve (for reference only)
Sheep alpha1-AGP ELISA Kit | alpha1-AGP elisa kit
Sheep alpha1-AGP (Alpha-1-Acid Glycoprotein) ELISA Kit
Gene Names
ORM2; AGP2; AGP-B; AGP-B'
Reactivity
Sheep
Synonyms
alpha1-AGP; Sheep alpha1-AGP (Alpha-1-Acid Glycoprotein) ELISA Kit; alpha1-AGP elisa kit
Reactivity
Sheep
Specificity
This kit recognizes SheepMMP-13in samples. No significantcross-reactivity or interference between SheepMMP-13 and analogues was observed.
Sequence Length
201
Samples
Serum, Plasma And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.094ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level SheepMMP-13 were tested 20times on one plate, respectively
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level SheepMMP-13 were testedon 3 different plates, 20 replicates in eachplate.
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for alpha1-AGP elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of SheepMMP-13 concentrations in ser um, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SheepMMP-13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SheepMMP-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SheepMMP-13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SheepMMP-13. You can calculate the concentration of SheepMMP-13 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SheepMMP-13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SheepMMP-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SheepMMP-13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SheepMMP-13. You can calculate the concentration of SheepMMP-13 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,603 Da
NCBI Official Full Name
alpha-1-acid glycoprotein 2
NCBI Official Synonym Full Names
orosomucoid 2
NCBI Official Symbol
ORM2
NCBI Official Synonym Symbols
AGP2; AGP-B; AGP-B'
NCBI Protein Information
alpha-1-acid glycoprotein 2; AGP 2; OMD 2; alpha-1-acid glycoprotein, type 2; orosomucoid-2
UniProt Protein Name
Alpha-1-acid glycoprotein 2
UniProt Gene Name
ORM2
UniProt Synonym Gene Names
AGP2; AGP 2; OMD 2
UniProt Entry Name
A1AG2_HUMAN
NCBI Description
This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however, it may be involved in aspects of immunosuppression. [provided by RefSeq, Jul 2008]
Uniprot Description
ORM2: Functions as transport protein in the blood stream. Binds various hydrophobic ligands in the interior of its beta- barrel domain. Also binds synthetic drugs and influences their distribution and availability. Appears to function in modulating the activity of the immune system during the acute-phase reaction. Belongs to the calycin superfamily. Lipocalin family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 9q32
Cellular Component: extracellular space
Biological Process: regulation of immune system process; transport; acute-phase response
Research Articles on alpha1-AGP
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Product Notes
The Sheep alpha1-AGP orm2 (Catalog #AAA2512819) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2512819 ELISA Kit recognizes Sheep alpha1-AGP. It is sometimes possible for the material contained within the vial of "alpha1-AGP, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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