Sheep Apolipoprotein (a) Antibody | anti-APOA2 antibody

Biotinylated Goat Anti-Human Apolipoprotein (a)

Cat. Num. AAA390006

• Gene Names: APOA2; apoAII; Apo-AII; ApoA-II
• Applications: ELISA, Immunoblot, Western Blot
• Synonyms: Apolipoprotein (a); Biotinylated Goat Anti-Human Apolipoprotein (a); Biotinylated Sheep Anti-Human Apolipoprotein (a) [Apo (a)]; Polyclonal Antibody; anti-APOA2 antibody

• Host: Sheep
• Specificity: Specific binds to human Lp (a) and apo (a). No cross reaction to apoB-100/48; minor to plasminogen. Dilution for immunoblot and ELISA range: 200 to 20,000
• Form/Format: 0.5 ml in Freeze-dried powder
• Concentration: 1.2 mg / ml (varies by lot)
• Sequence Length: 100

• Applicable Applications for anti-APOA2 antibody: ELISA (EIA), Immunoblot (IB)
• Application Notes: Freeze-dried powder. Dilution for Immunoblot & ELISA range: 1,000 to 20,000.; Use: The antibody can be used as second antibody in immunoblot or ELISA for detection of Lp (a) and apo (a) in human plasma and lipoproteins.
• Source Note: Polyclonal antibody purified by human apolipoprotein (a)- Sepharose affinity column and labeled with biotin. (Cross- reactivity to plasminogen was removed by passing plasminogen-Sepharose affinity column prior to apo (a)- Sepharose column.)
• Preservative: 50 mM PBS, 0.10 M NaCl, 0.5 mM EDTA, 0.02% NaN3, pH 7.4.
• Stabilizer: 10 mg / ml Bovine Serum Albumin.
• Preparation and Storage: Freeze-dried product should be stored refrigerated until opened. After opening, restore to 0.5 ml with distilled water. If it is not completely clear after standing for 1-2 Hours at room temperature, centrifuge the product. It is stable for several weeks at 4oC as an undiluted liquid. Do not use for more than one day after dilution. For extended storage after reconstitution, we suggest aliquot to avoid repeated freezing and thawing; or the addition of an equal volume of glycerol to make a final glycerol concentration of 50%, followed by storage at -20oC. The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol

Product Categories/Family for anti-APOA2 antibody

Biotin Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 671882
• NCBI GeneID: 336
• UniProt Accession #: P02652; B2R524
• Molecular Weight: 11,175 Da
• NCBI Official Full Name: apolipoprotein
• NCBI Official Synonym Full Names: apolipoprotein A-II
• NCBI Official Symbol: APOA2
• NCBI Official Synonym Symbols: apoAII; Apo-AII; ApoA-II
• NCBI Protein Information: apolipoprotein A-II; apolipoprotein A2
• UniProt Protein Name: Apolipoprotein A-II
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA2
• UniProt Synonym Gene Names: Apo-AII; ApoA-II
• UniProt Entry Name: APOA2_HUMAN

NCBI Description

This gene encodes apolipoprotein (apo-) A-II, which is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.

Subunit structure: Homodimer; disulfide-linked. Also forms a disulfide-linked heterodimer with APOD. Interacts with HCV core protein. Interacts with APOA1BP and NDRG1. Ref.15 Ref.16 Ref.19 Ref.23

Subcellular location: Secreted.

Tissue specificity: Plasma; synthesized in the liver and intestine.

Post-translational modification: Met-49 is oxidized to methionine sulfoxide.Phosphorylation sites are present in the extracellular medium.Apolipoprotein A-II is O-glycosylated. Ref.22

Polymorphism: A homozygous transition at position 1 of intron 3 of APOA2 results in deficiency of apolipoprotein A-II, without significant influence either on lipid and lipoprotein profiles or on the occurrence of coronary artery disease [

MIM:107670].

Sequence similarities: Belongs to the apolipoprotein A2 family.

Mass spectrometry: Molecular mass is 17252 Da from positions 24 - 100. Determined by ESI. Homodimer, without methionine sulfoxide. Ref.17Molecular mass is 17269 Da from positions 24 - 100. Determined by ESI. Homodimer, with 1 methionine sulfoxide, oxidation at Met-49. Ref.17Molecular mass is 8701.2 Da from positions 24 - 100. Determined by MALDI. Ref.18Molecular mass is 8823.4 Da from positions 24 - 100. Determined by MALDI. Cysteinylated ApoA-II. Ref.18Molecular mass is 17421.3 Da from positions 24 - 100. Determined by MALDI. Homodimer. Ref.18Molecular mass is 17293.4 Da from positions 24 - 100. Determined by MALDI. Heterodimer with truncated apolipoprotein A-II. Ref.18Molecular mass is 8578.3 Da from positions 24 - 99. Determined by MALDI. Ref.18Molecular mass is 17166.2 Da from positions 24 - 99. Determined by MALDI. Homodimer. Ref.18