SH2 Domain Containing Protein 1A (SH2D1A) Recombinant Protein | SH2D1A recombinant protein

Recombinant SH2 Domain Containing Protein 1A (SH2D1A)

Cat. Num. AAA2018634

• Gene Names: Sh2d1a; SAP; Gm686
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: SH2 Domain Containing Protein 1A (SH2D1A); Recombinant SH2 Domain Containing Protein 1A (SH2D1A); SH2D1A recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KLL LATGLDGSYL LRDSESVPGV YCLCVLYQGY IYTYRVSQTE TGSWSAETAP GVHKRFFRKV KNLISAFQKPDQGIVTPLQY PVEKSSGRGP QAPTG
• Sequence Length: 126

• Applicable Applications for SH2D1A recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Lys18~Gly115 (Accession # O88890) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Cytoplasm
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 9.4
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6755494
• NCBI GeneID: 20400
• NCBI Accession #: NP_035494.1
• NCBI GenBank Nucleotide #: NM_011364.3
• UniProt Accession #: O88890; Q9QWV6; A2ANL8
• Molecular Weight: 14.6kDa
• NCBI Official Full Name: SH2 domain-containing protein 1A
• NCBI Official Synonym Full Names: SH2 domain protein 1A
• NCBI Official Symbol: Sh2d1a
• NCBI Official Synonym Symbols: SAP; Gm686
• NCBI Protein Information: SH2 domain-containing protein 1A; Duncan disease homolog; SLAM-associated protein; T-cell signal transduction molecule SAP; signaling lymphocytic activation molecule-associated protein
• UniProt Protein Name: SH2 domain-containing protein 1A
• Protein Family: SH2 domain-containing protein
• UniProt Gene Name: Sh2d1a
• UniProt Synonym Gene Names: Xlp; SLAM-associated protein
• UniProt Entry Name: SH21A_MOUSE

Uniprot Description

SH2D1A: Inhibitor of the SLAM self-association. Acts by blocking recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to a docking site in the SLAM cytoplasmic region. Mediates interaction between FYN and SLAMF1. May also regulate the activity of the neurotrophin receptors NTRK1, NTRK2 and NTRK3. Interacts with NTRK1, NTRK2 and NTRK3. Interacts with CD84, CD244, LY9, SLAMF1 and FYN. Expressed at a high level in thymus and lung, with a lower level of expression in spleen and liver. Expressed in peripheral blood leukocytes, including T lymphocytes. Tends to be expressed at lower levels in peripheral blood leukocytes in patients with rheumatoid arthritis. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Adaptor/scaffold

Cellular Component: cytoplasm

Molecular Function: protein binding; SH3/SH2 adaptor activity

Biological Process: cell-cell signaling; cellular defense response; positive regulation of natural killer cell mediated cytotoxicity; humoral immune response