Surfactant Associated Protein B (SPB) Recombinant Protein | SPB recombinant protein

Recombinant Surfactant Associated Protein B (SPB)

Cat. Num. AAA2012244

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Surfactant Associated Protein B (SPB); Recombinant Surfactant Associated Protein B (SPB); SPB recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.
• Sequence: G AAITSASSLE CAQGPQFWCQ SLEHAVQCRA LGHCLQEVWG HAGANDLCQE CEDIVHLLTK MTKEDAFQEA IRKFLEQECD ILPLKLLVPR CRQVLDVYLP LVIDYFQSQI NPKAICNHVG LCPRGQAKPE QNPGMPDAVP NPLLDKLVLP VLPGALLARP GPHTQDFSEQ QLP
• Sequence Length: 377

• Applicable Applications for SPB recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Gly20~Pro193 (Accession # P50405) with N-terminal His-Tag
• Subcellular Location: Secreted, extracellular space, surface film.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Predicted isoelectric point: 5.8
• Usage/Reconstitution: Reconstitute in sterile ddH2O.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 1709875
• UniProt Accession #: P50405
• Molecular Weight: 20.7kDa
• NCBI Official Full Name: Pulmonary surfactant-associated protein B
• UniProt Protein Name: Pulmonary surfactant-associated protein B
• UniProt Gene Name: Sftpb
• UniProt Synonym Gene Names: Sftp3; SP-B
• UniProt Entry Name: PSPB_MOUSE

Uniprot Description

SFTPB: Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air- liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter. Defects in SFTPB are the cause of pulmonary surfactant metabolism dysfunction type 1 (SMDP1); also called pulmonary alveolar proteinosis due to surfactant protein B deficiency. A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid- Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Genetic variations in SFTPB are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS). RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'. A variation Ile to Thr at position 131 influences the association between specific alleles of SFTPA1 and respiratory distress syndrome in premature infants.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: multivesicular body; extracellular space; lysosome; cytoplasm; extracellular region

Biological Process: sphingolipid metabolic process; respiratory gaseous exchange; lipid metabolic process