Sheep 14-3-3 Protein sigma ELISA Kit | SFN elisa kit

Sheep 14-3-3 Protein sigma ELISA Kit

Cat. Num. AAA7266483

• Gene Names: SFN; YWHAS
• Reactivity: Sheep
• Synonyms: 14-3-3 Protein sigma; Sheep 14-3-3 Protein sigma ELISA Kit; SFN elisa kit

• Reactivity: Sheep
• Specificity: This assay has high sensitivity and excellent specificity for detection of YWHAS. No significant cross-reactivity or interference between YWHAS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between YWHAS and all the analogues, therefore, cross reaction may still exist in some cases.
• Sequence Length: 248

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store at 2-8 degree C.; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SFN elisa kit

Intended Uses: This YWHAS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of SheepYWHAS. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: YWHAS ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-YWHAS antibody and an YWHAS-HRP conjugate. The assay sample and buffer are incubated together with YWHAS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the YWHAS concentration since YWHAS from samples and YWHAS-HRP conjugate compete for the anti-YWHAS antibody binding site. Since the number of sites is limited, as more sites are occupied by YWHAS from the sample, fewer sites are left to bind YWHAS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YWHAS concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SFN elisa kit

Neuroscience; Signal Transduction; Stem Cells

NCBI and Uniprot Product Information

• NCBI GI #: 5454052
• NCBI GeneID: 2810
• NCBI Accession #: NP_006133.1
• NCBI GenBank Nucleotide #: NM_006142.5
• UniProt Accession #: P31947; Q6FH30; Q6FH51; Q96DH0
• Molecular Weight: 24,336 Da
• NCBI Official Full Name: 14-3-3 protein sigma
• NCBI Official Synonym Full Names: stratifin
• NCBI Official Symbol: SFN
• NCBI Official Synonym Symbols: YWHAS
• NCBI Protein Information: 14-3-3 protein sigma
• UniProt Protein Name: 14-3-3 protein sigma
• Protein Family: 14-3-3 protein
• UniProt Gene Name: SFN
• UniProt Synonym Gene Names: HME1

NCBI Description

This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis. [provided by RefSeq, Aug 2017]

Uniprot Description

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.