Vascular Endothelial Growth Factor Active Protein | VEGFA active protein

Vascular Endothelial Growth Factor, Recombinant, Mouse (VEGF)

Cat. Num. AAA650468

• Gene Names: VEGFA; VPF; VEGF; MVCD1; MGC70609
• Purity: Highly Purified; 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
• Synonyms: Vascular Endothelial Growth Factor; Recombinant; Mouse (VEGF); VEGFA active protein

• Host: Sf9 insect cells
• Purity/Purification: Highly Purified; 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
• Form/Format: Supplied as a lyophilized powder without additives.

• Biological Activity: Recombinant Mouse VEGF-Sf9 is fully biologically active when compared to standards. Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-2.0 ng/ml, corresponding to a specific activity of 1 x 106 Units/mg.
• Dimers and Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
• Endotoxin Level: 0.1ng/ug (IEU/ug) of VEGF-Sf9.
• Protein Content: Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280 nm.; 2. Analysis by RP-HPLC, using a calibrated solution of VEGF as a Reference Standard.
• Solubility: It is recommended to reconstitute the lyophilized VEGF-Sf9 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: -20 degree C; Stability: Lyophilized Recombinant VEGF-Sf9 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Murine VEGF-Sf9 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for VEGFA active protein

Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as Tumor Angiogenesis Factor or Vascular Permeability Factor. Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating Vasolidation via nitric oxide-dependent pathways. Alternative splicing of the mRNA for VEGF-A results in several isoforms of the protein being produced. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 percent. In contrast to other factors mitogenic for endothelial cells such as FGF-1, FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF.

Recombinant Mouse VEGF produced in Sf9 insect cells is a double, glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 38814 Dalton. Recombinant Murine VEGF is purified by proprietary chromatographic techniques.

Product Categories/Family for VEGFA active protein

Growth Factors; Cytokines; Growth Factors-VEGF

NCBI and Uniprot Product Information

• NCBI GI #: 181971
• NCBI GeneID: 7422
• UniProt Accession #: P15692; O60720; O75875; Q074Z4; Q16889; Q5UB46; Q6P0P5; Q96KJ0; Q96L82; Q96NW5; Q9H1W8; B5BU86; Q59FH5; Q6WZM0; Q71S09; Q96FD9; Q9UNS8
• Molecular Weight: 38.81kD
• NCBI Official Full Name: vascular endothelial growth factor
• NCBI Official Synonym Full Names: vascular endothelial growth factor A
• NCBI Official Symbol: VEGFA
• NCBI Official Synonym Symbols: VPF; VEGF; MVCD1; MGC70609
• NCBI Protein Information: vascular endothelial growth factor A; OTTHUMP00000016487; OTTHUMP00000016488; OTTHUMP00000165985; OTTHUMP00000165986; OTTHUMP00000165987; OTTHUMP00000224107; OTTHUMP00000224108; OTTHUMP00000224109; OTTHUMP00000224153; OTTHUMP00000224154; OTTHUMP00000224423; OTTHUMP00000224424; OTTHUMP00000224425; OTTHUMP00000224426; OTTHUMP00000224427; OTTHUMP00000224428; OTTHUMP00000224429; OTTHUMP00000224430; vascular permeability factor
• UniProt Protein Name: Vascular endothelial growth factor A
• Protein Family: Vascular endothelial growth factor
• UniProt Gene Name: VEGFA
• UniProt Synonym Gene Names: VEGF
• UniProt Entry Name: VEGFA_HUMAN

NCBI Description

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq]

Uniprot Description

Function: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Ref.27 Ref.30

Subunit structure: Homodimer; disulfide-linked. Also found as heterodimer with PGF

Subcellular location: Secreted. Note: VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. Ref.26 Ref.28 Ref.32

Tissue specificity: Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.

Induction: Regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoxia, hypoglycemia and oncogenic mutations.

Involvement in disease: Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [

MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.

Sequence similarities: Belongs to the PDGF/VEGF growth factor family.

Sequence caution: The sequence AAC63102.1 differs from that shown. Reason: Erroneous initiation. The sequence AAC63143.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence CAC19512.2 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.The sequence CAC19516.2 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.