Phosphoinositide 3 Kinase, p110 gamma Active Protein | PIK3R6 active protein

Phosphoinositide 3 Kinase, p110 gamma, Recombinant, Human (PI3K)

Cat. Num. AAA635708

• Gene Names: PIK3R6; p84; C17orf38; HsT41028; p87PIKAP; p84 PIKAP; p87(PIKAP)
• Purity: Highly Purified; 95% (SDS-PAGE)
• Synonyms: Phosphoinositide 3 Kinase; p110 gamma; Recombinant; Human (PI3K); PIK3R6 active protein

• Host: Sf9 insect cells
• Purity/Purification: Highly Purified; 95% (SDS-PAGE)
• Form/Format: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 50mM sodium chloride, 0.5mM MgCl2, 50% glycerol.

• Biological Activity: ~ 4.5 nmol/mg/min using phosphatidylinositol as the substrate.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for PIK3R6 active protein

PI3Kg is highly expressed in cells of hematopoietic origin. It plays an important role in dendritic cell (DC) trafficking and in the activation of specific immunity. PI3Kg-/- mice showed a reduced ability to respond to chemokines, and had a selective defect in the number of skin Langerhans cells and in lymph node CD8a-DCs. In macrophages, the chemokine RANTES/CCL5 activates the small GTPase Rac1 and its downstream target PAK2. This response depends on Gi activation and largely on the subsequent triggering of PI3Kg. Recombinant full length PI3Kg (126.3kD without Tag) carries a N-terminal His 8 affinity Tag.

Product Categories/Family for PIK3R6 active protein

Molecular Biology; MB-Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 58082081
• NCBI GeneID: 146850
• NCBI Accession #: NP_001010855.1
• NCBI GenBank Nucleotide #: NM_001010855.2
• UniProt Accession #: Q5UE93; Q658R3
• Molecular Weight: 126.3kD without Tag
• NCBI Official Full Name: phosphoinositide 3-kinase regulatory subunit 6
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, regulatory subunit 6
• NCBI Official Symbol: PIK3R6
• NCBI Official Synonym Symbols: p84; C17orf38; HsT41028; p87PIKAP; p84 PIKAP; p87(PIKAP)
• NCBI Protein Information: phosphoinositide 3-kinase regulatory subunit 6; p84 PI3K adapter protein; PI3Kgamma adapter protein of 87 kDa; p87 phosphoinositide 3-kinase gamma (PI3Kg) adapter protein
• UniProt Protein Name: Phosphoinositide 3-kinase regulatory subunit 6
• Protein Family: Phosphoinositide 3-kinase regulatory
• UniProt Gene Name: PIK3R6
• UniProt Synonym Gene Names: C17orf38; p84 PIKAP; p87PIKAP
• UniProt Entry Name: PI3R6_HUMAN

NCBI Description

Phosphoinositide 3-kinase gamma is a lipid kinase that produces the lipid second messenger phosphatidylinositol 3,4,5-trisphosphate. The kinase is composed of a catalytic subunit and one of several regulatory subunits, and is chiefly activated by G protein-coupled receptors. This gene encodes a regulatory subunit, and is distantly related to the phosphoinositide-3-kinase, regulatory subunit 5 gene which is located adjacent to this gene on chromosome 7. The orthologous protein in the mouse binds to both the catalytic subunit and to G(beta/gamma), and mediates activation of the kinase subunit downstream of G protein-coupled receptors. [provided by RefSeq, Jul 2008]

Uniprot Description

PIK3R6: Regulatory subunit of the PI3K gamma complex. Acts as an adapter to drive activation of PIK3CG by beta-gamma G protein dimers. The PIK3CG:PIK3R6 heterodimer is much less sensitive to beta-gamma G protein dimers than PIK3CG:PIK3R5 and its membrane recruitment and beta-gamma G protein dimer-dependent activation requires HRAS1 bound to PIK3CG. Recruits of the PI3K gamma complex to a PDE3B:RAPGEF3 signaling complex involved in angiogenesis; signaling seems to involve RRAS.

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: membrane; cytosol

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; 1-phosphatidylinositol-3-kinase regulator activity

Biological Process: platelet activation; G-protein coupled receptor protein signaling pathway; positive regulation of MAP kinase activity; positive regulation of angiogenesis; phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process; angiogenesis; blood coagulation; regulation of phosphoinositide 3-kinase activity