HDAC11 active protein

HDAC11 (human), (recombinant) (His-tag)

Cat. Num. AAA566699

• Purity: Partially purified by single-step affinity chromatography and gel filtration
• Synonyms: HDAC11; HDAC11 (human); (recombinant) (His-tag); HDAC11 active protein

• Host: Sf9 Insect cells
• Purity/Purification: Partially purified by single-step affinity chromatography and gel filtration
• Form/Format: Liquid. In 50mM TRIS, pH8.0, containing 138mM NaCl and 10% glycerol

• Source Note: Produced in a baculovirus expression system
• Biological Activity: Hydrolysis of N-epsilon-acetyl-lysine residues of histones
• Specific Activity: >=2U/ug. One unit is defined as the amount of enzyme that deacetylates 1 pmol/min of FLUOR DE LYS-SIRT1 substrate at 37 degree C / 500uM
• Preparation and Storage: For long term storage, store at -80 degree C; Shipping: Shipped on Dry Ice

Related Product Information for HDAC11 active protein

While the other ten human HDACs fall into either the class I (HDACs 1-3, 8) or class II (HDACs 4-7, 9, 10) homology groups, HDAC11 is the sole human representative in class IV.In contrast to the ubiquitous expression of most human HDACs, high level expression of HDAC11 is limited, in normal human tissues, to brain, heart, skeletal muscle, kidney and testis, suggesting possible tissue-specific or developmental functions. HDAC11 appears to play a role in neural cell maturation, particularly that of oligodendrocytes. HDAC11 deacetylation of histone H3 lysines 9 and 14 in the myelin basic protein and proteolipid protein genes stimulates their expression in oligodendrocytes, a notable example in which histone deacetylation, frequently associated with gene silencing, may in some cases have the opposite effect.  HDAC11 negatively regulates IL-10 expression in antigen presenting cells (APCs), thus influencing whether APCs induce T cell activation or tolerance. Elevated HDAC11 expression is seen in a number of cancer cell lines.  In myeloid leukemia lines, several HDAC inhibitors strongly induce HDAC11 expression, although the induction is more rapid with some inhibitors (TSA, SAHA) than others (butyrate, valproate).  Such effects may be important to consider in the development and clinical application of HDAC inhibitors as cancer therapeutics.

Product Categories/Family for HDAC11 active protein

Cellular Analysis; Cell Death; Stem Cells; Epigenetics; Histone Posttranslational Modifications; Proteostasis; Posttranslational Modifications; Proteolysis & Protein Degradation

NCBI and Uniprot Product Information

• UniProt Accession #: Q96DB2
• Molecular Weight: ~39 kDa
• UniProt Protein Name: Histone deacetylase 11
• Protein Family: Histone deacetylase
• UniProt Gene Name: HDAC11
• UniProt Synonym Gene Names: HD11

Uniprot Description

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.