HDAC10 active protein

HDAC10 (human), (recombinant) (His-tag)

Cat. Num. AAA566698

• Purity: Partially purified by single-step affinity chromatography and gel filtration
• Synonyms: HDAC10; HDAC10 (human); (recombinant) (His-tag); HDAC10 active protein

• Host: Sf9 Insect cells
• Purity/Purification: Partially purified by single-step affinity chromatography and gel filtration
• Form/Format: Liquid. In 50mM TRIS, pH8.0, containing 138mM NaCl and 10% glycerol

• Source Note: Produced in a baculovirus expression system
• Biological Activity: Hydrolysis of N-epsilon-acetyl-lysine residues of histones
• Specific Activity: >=2U/ug. One unit is defined as the amount of enzyme that deacetylates 1 pmol/min of FLUOR DE LYS-SIRT1 substrate at 37 degree C / 500uM
• Preparation and Storage: For long term storage, store at -80 degree C; Shipping: Shipped on Dry Ice

Related Product Information for HDAC10 active protein

HDAC10 is ubiquitously expressed in human tissues and many cell lines.  Its domain structure resembles that of its closest homolog and fellow class IIb HDAC, HDAC6, but rather than two active deacetylase domains, it has one active domain (N-terminal) and a partially duplicated, inactive, C-terminal domain. Also like HDAC6, HDAC10 is resistant to inhibition by trapoxin B, sodium butyrate and valproic acid. Inhibition of one or both of the class IIb HDACs may be a desirable goal for cancer therapy.  Inhibition or siRNA knockdown of both HDAC10 and HDAC6 causes depletion of the VEGFR1 and VEGFR2 proteins, suggesting a strategy for blocking angiogenesis via HDAC6/HDAC10 inhibition.  An HDAC10 promoter polymorphism that increases transcription is associated with increased occurence of hepatocellular carcinoma in individuals with chronic hepatitis B infections.

Product Categories/Family for HDAC10 active protein

Cellular Analysis; Cell Death; Stem Cells; Epigenetics; Histone Posttranslational Modifications; Proteostasis; Posttranslational Modifications; Proteolysis & Protein Degradation

NCBI and Uniprot Product Information

• UniProt Accession #: Q969S8
• Molecular Weight: ~71 kDa
• UniProt Protein Name: Histone deacetylase 10
• Protein Family: Histone deacetylase
• UniProt Gene Name: HDAC10
• UniProt Synonym Gene Names: HD10
• UniProt Entry Name: HDA10_HUMAN

Uniprot Description

HDAC10: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Belongs to the histone deacetylase family. HD type 2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Deacetylase; EC 3.5.1.98; Transcription factor

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: nucleoplasm; histone deacetylase complex; cytoplasm; nucleus

Molecular Function: protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); histone deacetylase binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity

Biological Process: Notch signaling pathway; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; protein amino acid deacetylation; regulation of transcription, DNA-dependent; histone deacetylation; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; oligodendrocyte development; negative regulation of transcription, DNA-dependent