CD152, Fc Chimera Active Protein
CD152, Fc Chimera, Recombinant, Human (CTLA4, Cytotoxic T-lymphocyte-associated Antigen 4)
Purity
Highly Purified95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. Endotoxin: 0.1ng/ug (IEU/ug) of CTLA-4.
Synonyms
CD152; Fc Chimera; Recombinant; Human (CTLA4; Cytotoxic T-lymphocyte-associated Antigen 4); Fc Chimera active protein
Host
Sf9 Insect cells
Purity/Purification
Highly Purified
95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. Endotoxin: 0.1ng/ug (IEU/ug) of CTLA-4.
95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. Endotoxin: 0.1ng/ug (IEU/ug) of CTLA-4.
Form/Format
Supplied as a lyophilized powder without additives. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. to a concentration of >0.1mg/ml. This solution can then be diluted into other aqueous solutions.
Sequence
The sequence of the amino acids was determined and was found to be: Lys-Ala-Met-His-Val.
Biological Activity
Recombinant CTLA-4 is fully biologically active when compared to standards.
Dimers and Aggregates
1% as determined by silver-stained SDS-PAGE gel analysis.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280.
2. Analysis by RP-HPLC, using a standard solution of Human CTLA-4 as a Reference Standard.
1. UV spectroscopy at 280.
2. Analysis by RP-HPLC, using a standard solution of Human CTLA-4 as a Reference Standard.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% HSA or BSA. aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Related Product Information for CD152, Fc Chimera active protein
CTLA-4 is expressed in low copy number by T-cells only after activation, but it binds CD28-ligand with approximately 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind CD28-ligand with high avidity and to suppress T-cell-dependent antibody responses in vivo. Large doses of this soluble protein also suppress responses to a second immunization.
Product Categories/Family for CD152, Fc Chimera active protein
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Product Notes
The CD152, Fc Chimera (Catalog #AAA636307) is an Active Protein produced from Sf9 Insect cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the amino acids was determined and was found to be: Lys-Ala-Me t-His-Val. It is sometimes possible for the material contained within the vial of "CD152, Fc Chimera, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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