Human Sestrin-3 ELISA Kit | SESN3 elisa kit

Human Sestrin-3 ELISA Kit

Cat. Num. AAA762304

• Gene Names: SESN3; SEST3
• Reactivity: Human
• Synonyms: Sestrin-3; Human Sestrin-3 ELISA Kit; SESN3 (Sestrin-3)/SEST3; SESN3 elisa kit

• Reactivity: Human
• Sequence Length: 353

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for SESN3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- SESN3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- SESN3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SESN3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SESN3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 409971404
• NCBI GeneID: 143686
• NCBI Accession #: NP_001258523.1
• NCBI GenBank Nucleotide #: NM_001271594.1
• Molecular Weight: 41,522 Da
• NCBI Official Full Name: sestrin-3 isoform 2
• NCBI Official Synonym Full Names: sestrin 3
• NCBI Official Symbol: SESN3
• NCBI Official Synonym Symbols: SEST3
• NCBI Protein Information: sestrin-3
• UniProt Protein Name: Sestrin-3
• Protein Family: Sestrin
• UniProt Gene Name: SESN3
• UniProt Synonym Gene Names: SEST3
• UniProt Entry Name: SESN3_HUMAN

NCBI Description

This gene encodes a member of the sestrin family of stress-induced proteins. The encoded protein reduces the levels of intracellular reactive oxygen species induced by activated Ras downstream of RAC-alpha serine/threonine-protein kinase (Akt) and FoxO transcription factor. The protein is required for normal regulation of blood glucose, insulin resistance and plays a role in lipid storage in obesity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]

Uniprot Description

SESN3: a member of the sestrin family of stress-induced proteins. The encoded protein reduces the levels of intracellular reactive oxygen species induced by activated Ras downstream of RAC-alpha serine/threonine-protein kinase (Akt) and FoxO transcription factor. The protein is required for normal regulation of blood glucose, insulin resistance and plays a role in lipid storage in obesity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: nucleus

Biological Process: glucose homeostasis; regulation of protein kinase B signaling cascade; response to insulin stimulus