SERPINH1 (Hsp47) Recombinant Protein | SERPINH1 recombinant protein

Recombinant Human SERPINH1 (Hsp47)

Cat. Num. AAA197050

• Gene Names: SERPINH1; CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: SERPINH1 (Hsp47); Recombinant Human SERPINH1 (Hsp47); SERPINH1 recombinant protein

• Host: E Coli
• Specificity: Human SERPINH1 (Hsp47)
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: Sterile-filtered colorless solution (1mg/ml) in 20mM Tris-HCl, pH 8.0, and 10% glycerol. Purified by proprietary chromatographic techniques.
• Sequence: RGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAA EVKKPAAAAA GTAEKLSPK AATLAERSAG LAFSLYQAMA KDQAVENILV SPVVVASSLG LVSLGGKATT ASQAKAVLSA EQLRDEEVHA GLGELLRSLS NSTARNVTWK LGSRLYGPSS VSFADDFVRS SKQHYNCEHS KINFRDKRSA LQSINEWAAQ TTDGKLPEVT KDVERTDGAL LVNAMFFKPH WDEKFHHKMV DNRGFMVTRS YTVGVMMMHR TGLYNYYDDE KEKLQIVEMP LAHKLSSLII LMPHHVEPLE RLEKLLTKEQ LKIWMGKMQK KAVAISLPKG VVEVTHDLQK HLAGLGLTEA IDKNKADLSR MSGKKDLYLA SVFHATAFEL DTDGNPFDQD IYGREELRSP KLFYADHPFI FLVRDTQSGS LLFIGRLVRP KGDKMRDEL.

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store at or below -20 degree C for longer periods of time. Addition of a carrier protein (such as 0.1% HSA or BSA) is recommended for long-term storage.

Related Product Information for SERPINH1 recombinant protein

SERPINH1 is a member of the serpin superfamily of serine proteinase inhibitors whose expression is induced by heat shock. As a molecular chaperone, it localizes to the endoplasmic reticulum lumen and binds collagen, thus participating in the maturation of collagen molecules, facilitating the folding and assembly of procollagen molecules, retaining unfolded molecules within the ER, and assisting in the transport of correctly folded molecules from the ER to the Golgi apparatus. Autoantibodies to SERPINH1 have been found in cases of rheumatoid arthritis.

Recombinant human SERPINH1 produced in E. coli is a single, nonglycosylated polypeptide containing 439 amino acids (aa 18-417) with a molecular weight of 48.9kDa. It is fused to a 37- amino acid His-tag at the Nterminus.

NCBI and Uniprot Product Information

• NCBI GI #: 333360851
• NCBI GeneID: 871
• NCBI Accession #: NP_001193943.1
• NCBI GenBank Nucleotide #: NM_001207014.1
• UniProt Accession #: P50454; P29043; Q5XPB4; Q6NSJ6; Q8IY96; Q9NP88; B3KVJ3; Q9NPA9
• Molecular Weight: 46,441 Da
• NCBI Official Full Name: serpin H1
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
• NCBI Official Symbol: SERPINH1
• NCBI Official Synonym Symbols: CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2
• NCBI Protein Information: serpin H1; colligin-1; colligin-2; OTTHUMP00000229451; OTTHUMP00000229452; OTTHUMP00000229453; OTTHUMP00000229454; OTTHUMP00000229458; 47 kDa heat shock protein; arsenic-transactivated protein 3; rheumatoid arthritis antigen A-47; cell proliferation-inducing gene 14 protein; rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1); serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2)
• UniProt Protein Name: Serpin H1
• Protein Family: Serpin
• UniProt Gene Name: SERPINH1
• UniProt Synonym Gene Names: CBP1; CBP2; HSP47; SERPINH2
• UniProt Entry Name: SERPH_HUMAN

NCBI Description

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq]

Uniprot Description

SERPINH1: Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen. Defects in SERPINH1 are the cause of osteogenesis imperfecta type 10 (OI10). A connective tissue disorder characterized by bone fragility, low bone mass, bowing of limbs due to multiple fractures, short limb dwarfism and blue sclerae. Belongs to the serpin family.

Chromosomal Location of Human Ortholog: 11q13.5

Cellular Component: extracellular space; endoplasmic reticulum lumen; endoplasmic reticulum; ER-Golgi intermediate compartment

Molecular Function: serine-type endopeptidase inhibitor activity; collagen binding; unfolded protein binding

Biological Process: extracellular matrix organization and biogenesis; collagen fibril organization; protein maturation; collagen biosynthetic process; response to unfolded protein

Disease: Osteogenesis Imperfecta, Type X; Preterm Premature Rupture Of The Membranes