SERPINE1 recombinant protein

Recombinant Mouse SERPINE1 Protein

Cat. Num. AAA8249775

• Gene Names: Serpine1; PAI1; PAI-1; Planh1
• Reactivity: Mouse
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: SERPINE1; Recombinant Mouse SERPINE1 Protein; PAI1; PLANH1; Plasminogen activator inhibitor 1; PAI; PAI-1; Endothelial plasminogen activator inhibitor; Serpin E1; SERPINE1 recombinant protein

• Host: Human cells
• Reactivity: Mouse
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Sequence: LPLRESHTAH QATDFGVKVF QQVVQASKDR NVVFSPYGVS SVLAMLQMTT AGKTRRQIQD AMGFKVNEKG TAHALRQLSK ELMGPWNKNE ISTADAIFVQ RDLELVQGFM PHFFKLFQTM VKQVDFSEVE RARFIINDWV ERHTKGMISD LLAKGAVDEL TRLVLVNALY FSGQWKTPFL EASTHQRLFH KSDGSTVSVP MMAQSNKFNY TEFTTPDGLE YDVVELPYQG DTLSMFIAAP FEKDVHLSAL TNILDAELIR QWKGNMTRLP RLLILPKFSL ETEVDLRGPL EKLGMPDMFS ATLADFTSLS DQEQLSVAQA LQKVRIEVNE SGTVASSSTA FVISARMAPT EMVIDRSFLF VVRHNPTETI LFMGQVMEPV DHHHHHH
• Sequence Length: 402

• Applicable Applications for SERPINE1 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for SERPINE1 recombinant protein

Recombinant Mouse SERPINE1 Protein is produced by our mammalian expression system and the target gene encoding Leu24-Ser402 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 129577
• NCBI GeneID: 18787
• UniProt Accession #: P22777
• Molecular Weight: 45,170 Da
• NCBI Official Full Name: Plasminogen activator inhibitor 1
• NCBI Official Synonym Full Names: serine (or cysteine) peptidase inhibitor, clade E, member 1
• NCBI Official Symbol: Serpine1
• NCBI Official Synonym Symbols: PAI1; PAI-1; Planh1
• NCBI Protein Information: plasminogen activator inhibitor 1
• UniProt Protein Name: Plasminogen activator inhibitor 1
• Protein Family: Plasminogen activator inhibitor
• UniProt Gene Name: Serpine1
• UniProt Synonym Gene Names: Mr1; Pai1; Planh1; PAI; PAI-1
• UniProt Entry Name: PAI1_MOUSE

Uniprot Description

SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Cellular Component: cytoplasm; extracellular matrix; extracellular region; extracellular space

Molecular Function: protease binding; protein binding; receptor binding; serine-type endopeptidase inhibitor activity

Biological Process: defense response to Gram-negative bacterium; female gonad development; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; negative regulation of fibrinolysis; negative regulation of smooth muscle cell migration; placenta development; positive regulation of angiogenesis; positive regulation of blood coagulation; positive regulation of coagulation; positive regulation of fibroblast proliferation; positive regulation of inflammatory response; positive regulation of interleukin-8 production; positive regulation of keratinocyte migration; positive regulation of receptor-mediated endocytosis; regulation of angiogenesis; regulation of cell proliferation; regulation of receptor activity; wound healing