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SERPINB4 recombinant protein
SERPINB4 Protein, Human, Recombinant (His Tag)
Gene Names
SERPINB4; PI11; SCCA1; SCCA2; LEUPIN; SCCA-2
Purity
>88% as determined by SDS-PAGE
Synonyms
SERPINB4; SERPINB4 Protein; Human; Recombinant (His Tag); Human SerpinB4 Protein (His Tag); LEUPIN Protein; PI11 Protein; SCCA-2 Protein; SCCA1 Protein; SCCA2 Protein; Serpin B4 Protein; serpin peptidase inhibitor; clade B (ovalbumin); member 4; SERPINB4 recombinant protein
Host
Baculovirus-Insect Cells
Purity/Purification
>88% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 20% gly, 1mM EDTA, 1mM DTT. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA
Sequence
Met1-Pro390
Species
Human
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Met
Tag
N-His
Protein Construction
A DNA sequence encoding the human SERPINB4 (P48594)(Met 1-Pro 390) was expressed, with a polyhistidine tag at the N-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for SERPINB4 recombinant protein
Background: Overexpression of SERPINB4 in tumor cells inhibited recombinant GrM-induced as well as NK cell-mediated cell death and this inhibition depended on the reactive center loop of the serpin.
References
de Koning PJ, et al. (2011) Intracellular Serine Protease Inhibitor SERPINB4 Inhibits Granzyme M-Induced Cell Death. PLoS One. 6(8): e22645.Chechlinska M, et al. (2010) Squamous cell carcinoma antigen 1 and 2 expression in cultured normal peripheral blood mononuclear cells and in vulvar squamous cell carcinoma. Tumour Biol. 31(6): 559-67.Shiiba M, et al. (2010) Down-regulated expression of SERPIN genes located on chromosome 18q21 in oral squamous cell carcinomas. Oncol Rep. 24(1): 241-9. en, Der p 1. J Biol Chem. 279(7): 5081-7.Murakami A, et al. (2008) Relationship between decreased expression of squamous cell carcinoma antigen 2 and E-cadherin in primary cervical cancer lesions and lymph node metastasis. Oncol Rep. 19(1): 99-104.Silverman GA, et al. (1998) SCCA1 and SCCA2 are proteinase inhibitors that map to the serpin cluster at 18q21.3. Tumour Biol. 19(6): 480-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,854 Da
NCBI Official Full Name
serpin B4
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade B (ovalbumin), member 4
NCBI Official Symbol
SERPINB4
NCBI Official Synonym Symbols
PI11; SCCA1; SCCA2; LEUPIN; SCCA-2
NCBI Protein Information
serpin B4; PI-11; peptidase inhibitor 11; SCCA2/SCCA1 fusion protein; squamous cell carcinoma antigen 1; squamous cell carcinoma antigen 2; protease inhibitor (leucine-serpin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 4
UniProt Protein Name
Serpin B4
Protein Family
UniProt Gene Name
SERPINB4
UniProt Synonym Gene Names
PI11; SCCA2; PI-11; SCCA-2
UniProt Entry Name
SPB4_HUMAN
Uniprot Description
SERPINB4: May act as a protease inhibitor to modulate the host immune response against tumor cells. Belongs to the serpin family. Ov-serpin subfamily.
Protein type: Inhibitor
Chromosomal Location of Human Ortholog: 18q21.3
Cellular Component: extracellular space; cytoplasm; intracellular
Molecular Function: serine-type endopeptidase inhibitor activity; enzyme binding; protease binding
Biological Process: regulation of proteolysis; negative regulation of peptidase activity; protection from natural killer cell mediated cytotoxicity
Research Articles on SERPINB4
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Product Notes
The SERPINB4 serpinb4 (Catalog #AAA8121243) is a Recombinant Protein produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Pro39 0. It is sometimes possible for the material contained within the vial of "SERPINB4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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