Serpin A1/alpha-1-Antitrypsin Recombinant Protein | SERPINA1 recombinant protein

Recombinant Human Serpin A1/alpha-1-Antitrypsin Protein (C-6His)

Cat. Num. AAA2553210

• Gene Names: SERPINA1; PI; A1A; AAT; PI1; A1AT; nNIF; PRO2275; alpha1AT
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Serpin A1/alpha-1-Antitrypsin; Recombinant Human Serpin A1/alpha-1-Antitrypsin Protein (C-6His); Alpha-1-Antitrypsin; Alpha-1 Protease Inhibitor; Alpha-1-Antiproteinase; Serpin A1; SERPINA1; AAT; PI; SERPINA1 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 20mM Tris HCl, 150mM NaCl, 2mM CaCl2, pH 7.5.
• Sequence Positions: Glu25-Lys418
• Sequence Length: 418

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fusion Tag: C-6His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for SERPINA1 recombinant protein

Serpin A1 is a prototype member of the Serpin superfamily of the serine protease inhibitors. As one of the most abundant proteinase inhibitors in the circulation, it is synthesized in hepatocytes, and to a lesser extent, in macrophages as well as intestinal epithelial cell lines and secreted as the abundant proteinase inhibitor in the circulation whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Point mutations in the native SerpinA1 variants result in Serpin A1 deficiency, and consequently lead to several clinical complications such as pulmonary emphysema, juvenile hepatitis, cirrhosis, and hepatocellular carcinoma. For example, the Z variants (Glu342 to Lys) forms intracellular inclusion bodies, is not secreted, and leads to a severe SerpinA1 deficiency. Accordingly, Serpin A1 deficiency in circulation is associated with emphysema or liver disease.

NCBI and Uniprot Product Information

• NCBI GI #: 50363217
• NCBI GeneID: 5265
• NCBI Accession #: NP_000286.3
• NCBI GenBank Nucleotide #: NM_000295.4
• UniProt Accession #: P01009; Q0PVP5; Q13672; Q53XB8; Q5U0M1; Q7M4R2; Q86U18; Q86U19; Q96BF9; Q96ES1; A6PX14; B2RDQ8
• Molecular Weight: Molecular Mass: 45.4 kDa; Actual Protein Molecular Mass: 54 kDa
• NCBI Official Full Name: alpha-1-antitrypsin
• NCBI Official Synonym Full Names: serpin family A member 1
• NCBI Official Symbol: SERPINA1
• NCBI Official Synonym Symbols: PI; A1A; AAT; PI1; A1AT; nNIF; PRO2275; alpha1AT
• NCBI Protein Information: alpha-1-antitrypsin
• UniProt Protein Name: Alpha-1-antitrypsin
• Protein Family: Alpha-1-antitrypsin
• UniProt Gene Name: SERPINA1
• UniProt Synonym Gene Names: AAT; PI; SPAAT

NCBI Description

The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.