Mouse Semaphorin-4D ELISA Kit | SEMA4D elisa kit

Mouse Semaphorin-4D ELISA Kit

Cat. Num. AAA14536

• Gene Names: SEMA4D; CD100; SEMAJ; coll-4; C9orf164; M-sema-G
• Reactivity: Mouse
• Synonyms: Semaphorin-4D; Mouse Semaphorin-4D ELISA Kit; SEMA4D elisa kit

• Reactivity: Mouse

• Samples: Serum, Plasma, Cell Culture Supernatants, Tissue Homogenate, Urine and other Body Fluids
• Detection Range: 125 - 4000 pg/ml.
• Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Mouse SEMA4D in the specimen, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the specimens and allow the operator to produce a standard curve of Optical Density versus Mouse SEMA4D concentration. The concentration of Mouse SEMA4D in the specimens is then determined by comparing the O.D. of the specimens to the standard curve.
• Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Mouse SEMA4D in the specimen, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the specimens and allow the operator to produce a standard curve of Optical Density versus Mouse SEMA4D concentration. The concentration of Mouse SEMA4D in the specimens is then determined by comparing the O.D. of the specimens to the standard curve.
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for SEMA4D elisa kit

Background: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of specimens. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the specimen.

NCBI and Uniprot Product Information

• NCBI GI #: 214010218
• NCBI GeneID: 10507
• NCBI Accession #: NP_006369.3
• NCBI GenBank Nucleotide #: NM_006378.3
• UniProt Accession #: Q92854; Q7Z5S4; Q8N8B0; B2RPM6
• Molecular Weight: 96,150 Da
• NCBI Official Full Name: semaphorin-4D isoform 1
• NCBI Official Synonym Full Names: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
• NCBI Official Symbol: SEMA4D
• NCBI Official Synonym Symbols: CD100; SEMAJ; coll-4; C9orf164; M-sema-G
• NCBI Protein Information: semaphorin-4D; A8; GR3; BB18; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D
• UniProt Protein Name: Semaphorin-4D
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA4D
• UniProt Synonym Gene Names: C9orf164; CD100; SEMAJ
• UniProt Entry Name: SEM4D_HUMAN

Uniprot Description

SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q22.2

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor activity; semaphorin receptor binding; receptor binding

Biological Process: axon guidance; negative regulation of peptidyl-tyrosine phosphorylation; regulation of dendrite morphogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell adhesion; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell shape; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of osteoblast differentiation; immune response; positive regulation of protein amino acid phosphorylation; cell adhesion; positive regulation of collateral sprouting; negative regulation of apoptosis; regulation of cell projection organization and biogenesis; positive regulation of cell migration