SEMA4C recombinant protein

Recombinant Mouse SEMA4C Protein

Cat. Num. AAA8249748

• Gene Names: Sema4c; Semaf; Semai; sema I; Semacl1; AI426163; M-Sema F
• Reactivity: Mouse
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: SEMA4C; Recombinant Mouse SEMA4C Protein; KIAA1739; SEMAI; Semaphorin-4C; SEMA4C recombinant protein

• Host: Human cells
• Reactivity: Mouse
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of PBS, pH 7.4.
• Sequence: AEMWWNLVPR KTVSSGELVT VVRRFSQTGI QDFLTLTLTE HSGLLYVGAR EALFAFSVEA LELQGAISWE APAEKKIECT QKGKSNQTEC FNFIRFLQPY NSSHLYVCGT YAFQPKCTYI NMLTFTLDRA EFEDGKGKCP YDPAKGHTGL LVDGELYSAT LNNFLGTEPV ILRYMGTHHS IKTEYLAFWL NEPHFVGSAF VPESVGSFTG DDDKIYFFFS ERAVEYDCYS EQVVARVARV CKGDMGGART LQKKWTTFLK ARLVCSAPDW KVYFNQLKAV HTLRGASWHN TTFFGVFQAR WGDMDLSAVC EYQLEQIQQV FEGPYKEYSE QAQKWARYTD PVPSPRPGSC INNWHRDNGY TSSLELPDNT LNFIKKHPLM EDQVKPRLGR PLLVKKNTNF THVVADRVPG LDGATYTVLF IGTGDGWLLK AVSLGPWIHM VEELQVFDQE PVESLVLSQS KKVLFAGSRS QLVQLSLADC TKYRFCVDCV LARDPYCAWN VNTSRCVATT SGRSGSFLVQ HVANLDTSKM CNQYGIKKVR SIPKNITVVS GTDLVLPCHL SSNLAHAHWT FGSQDLPAEQ PGSFLYDTGL QALVVMAAQS RHSGPYRCYS EEQGTRLAAE SYLVAVVAGS SVTLEARAPL ENLGVDDIEG RMDEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSREEMTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGK
• Sequence Length: 834

• Applicable Applications for SEMA4C recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for SEMA4C recombinant protein

Recombinant Mouse SEMA4C Protein is produced by our mammalian expression system and the target gene encoding Ala21-Gly664 is expressed with a Fc tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 186659517
• NCBI GeneID: 20353
• NCBI Accession #: NP_001119519.1
• NCBI GenBank Nucleotide #: NM_001126047.3
• UniProt Accession #: Q64151
• Molecular Weight: 92,557 Da
• NCBI Official Full Name: semaphorin-4C
• NCBI Official Synonym Full Names: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C
• NCBI Official Symbol: Sema4c
• NCBI Official Synonym Symbols: Semaf; Semai; sema I; Semacl1; AI426163; M-Sema F
• NCBI Protein Information: semaphorin-4C
• UniProt Protein Name: Semaphorin-4C
• Protein Family: Semaphorin
• UniProt Gene Name: Sema4c
• UniProt Synonym Gene Names: S4c; Semacl1; Semai; Sema I; Semaphorin I
• UniProt Entry Name: SEM4C_MOUSE

NCBI Description

This gene encodes a member of the semaphorin family of proteins that have diverse functions in neuronal development, heart morphogenesis, vascular growth, tumor progression and immune cell regulation. Lack of the encoded protein in some mice causes exencephaly resulting in neonatal lethality. Mice that bypass exencephaly show no obvious behavioral defects but display distinct pigmentation defects. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

SEMA4C: Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration. Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade. Belongs to the semaphorin family.

Protein type: Membrane protein, integral

Cellular Component: extracellular space; postsynaptic density; synaptic vesicle membrane

Molecular Function: chemorepellent activity; protein binding; semaphorin receptor binding

Biological Process: cell migration in hindbrain; cerebellum development; muscle cell differentiation; negative chemotaxis; negative regulation of axon extension involved in axon guidance; neural crest cell migration; neural tube closure; positive regulation of cell migration; positive regulation of stress-activated MAPK cascade